Microfluidic multi-angle laser scattering system for rapid and label-free detection of waterborne parasites.

A microfluidic laser scattering system for rapid and label-free detection of single waterborne parasites in microfluidic flows was designed, fabricated and demonstrated. The key novelty of the system lies in the integration of functional modules involving pre-concentration, on-chip laser scattering detection, and pattern recognition. The silicon-based pre-concentration chip can concentrate 10 ml reagent water sample spiked with protozoa (oo)cysts into a volume of 200 μl in ~30 minutes. The concentrated sample is further channeled into the on-chip laser scattering detection module at a flow rate of 10 μl/min, which can collect the multi-angle scattering pattern of single flowing microparticles. The Zernike moment features of scattering patterns are extracted using principal component analysis, and classification of scattering patterns are performed using the linear discriminator analysis algorithm. As a result, Cryptosporidium parvum oocysts and Giardia lamblia cysts spiked in ~10 ml reagent water can be enumerated and identified within an hour without labeling, with a mean recovery efficiency of ~73% and average accuracies of 96%, 97%, 97% and 98% at concentrations of 10, 50, 100, 300 (oo)cysts per 10 ml water, respectively. We believe that this compact microfluidic laser scattering system has potential for rapid and label-free water quality monitoring in field and resource-limited environments.