Madhu Kaundal1, Rahul Deshmukh1, Mohd Akhtar2. 1. Department of Pharmacology, School of Pharmaceutical Education and Research Formerly, Faculty of Pharmacy, Jamia Hamdard (Hamdard University), New Delhi, India. 2. Department of Pharmacology, School of Pharmaceutical Education and Research Formerly, Faculty of Pharmacy, Jamia Hamdard (Hamdard University), New Delhi, India. Electronic address: makhtar_ph@jamiahamdard.ac.in.
Abstract
BACKGROUND: The purpose of the study was to explore the therapeutic potential of Betulinic acid (BA) in streptozotocin (STZ) induced memory damage in experimental rats. METHODS: STZ (3mg/kg bilaterally) as intracerebroventrical (icv) route was administered on day 1 and 3 in rats. Donepezil (5mg/kg/day po), used as standard, and BA (5, 10 and 15mg/kg/day po) were administered after 1h of 1st STZ infusion up to 21days. Object recognition task (ORT) for non-spatial, Morris water maze (MWM) for spatial and locomotor activity were performed to evaluate behavioral changes in rats. On 22nd day, animals were decapitated and hippocampus was separated to perform biochemical (AChE, LPO, GSH, nitrite), neuroinflammatory (TNF-α, IL-1β, and IL-6), neurotransmitters (NTs) (dopamine, norepinephrine and serotonin) analysis. RESULTS: STZ infusion significantly impaired memory as observed in MWM and ORT, increased oxidative stress, pro-inflammatory cytokine's level and altered NTs level. Moreover, BA demonstrated a neuroprotective effect in a dose-dependent manner. BA dose dependently (5, 10 and 15mg/kg) significantly restore STZ induced memory changes and pathological abnormalities in rat brain. CONCLUSIONS: The findings of the current study suggests that BA protect rat brain from STZ induced neuronal damage via acting through multiple mechanisms and would be used to curb cognitive decline associated with neurodegenerative disorders especially AD.
BACKGROUND: The purpose of the study was to explore the therapeutic potential of Betulinic acid (BA) in streptozotocin (STZ) induced memory damage in experimental rats. METHODS:STZ (3mg/kg bilaterally) as intracerebroventrical (icv) route was administered on day 1 and 3 in rats. Donepezil (5mg/kg/day po), used as standard, and BA (5, 10 and 15mg/kg/day po) were administered after 1h of 1st STZ infusion up to 21days. Object recognition task (ORT) for non-spatial, Morris water maze (MWM) for spatial and locomotor activity were performed to evaluate behavioral changes in rats. On 22nd day, animals were decapitated and hippocampus was separated to perform biochemical (AChE, LPO, GSH, nitrite), neuroinflammatory (TNF-α, IL-1β, and IL-6), neurotransmitters (NTs) (dopamine, norepinephrine and serotonin) analysis. RESULTS:STZ infusion significantly impaired memory as observed in MWM and ORT, increased oxidative stress, pro-inflammatory cytokine's level and altered NTs level. Moreover, BA demonstrated a neuroprotective effect in a dose-dependent manner. BA dose dependently (5, 10 and 15mg/kg) significantly restore STZ induced memory changes and pathological abnormalities in rat brain. CONCLUSIONS: The findings of the current study suggests that BA protect rat brain from STZ induced neuronal damage via acting through multiple mechanisms and would be used to curb cognitive decline associated with neurodegenerative disorders especially AD.
Authors: Jelena Osmanovic Barilar; Ana Knezovic; Ana Babic Perhoc; Jan Homolak; Peter Riederer; Melita Salkovic-Petrisic Journal: J Neural Transm (Vienna) Date: 2020-02-06 Impact factor: 3.575
Authors: Ashraf Al-Brakati; Alaa Jameel A Albarakati; Hamid M A Daabo; Roua S Baty; Fatma Elzahraa H Salem; Ola A Habotta; Ehab K Elmahallawy; Doaa M Abdel-Mohsen; Heba Taha; Ahmed M A Akabawy; Rami B Kassab; Ahmed E Abdel Moneim; Hatim K Amin Journal: Metab Brain Dis Date: 2020-06-16 Impact factor: 3.584
Authors: María E Prados; Alejandro Correa-Sáez; Juan D Unciti-Broceta; Martín Garrido-Rodríguez; Carla Jimenez-Jimenez; Massimiliano Mazzone; Alberto Minassi; Giovanni Appendino; Marco A Calzado; Eduardo Muñoz Journal: Neurotherapeutics Date: 2021-08-02 Impact factor: 7.620