Literature DB >> 2967073

Lp(a) phenotyping by immunoblotting with polyclonal and monoclonal antibodies.

H G Kraft1, H Dieplinger, E Hoye, G Utermann.   

Abstract

A new method that allows rapid phenotyping of genetic Lp(a) glycoprotein types in large numbers of samples is described. The method is based on sodium dodecyl sulfate gel electrophoresis of reduced serum or plasma in horizontal slab gels followed by immunoblotting with polyclonal anti-Lp(a) lipoprotein or monoclonal anti-Lp(a) glycoprotein antibodies. Phenotyping of 194 unrelated, healthy subjects resulted in Lp(a) allele frequencies of Lp(a)B = 0.013, Lp(a)S1 = 0.032, Lp(a)S2 = 0.106, Lp(a)S3 = 0.096, Lp(a)S4 = 0.156, and Lp(a)O = 0.600, and confirmed the recently recognized association of Lp(a) glycoprotein phenotype with Lp(a) lipoprotein concentration. The new procedure is suitable for large-scale population, genetic, and epidemiologic studies and may be important for atherosclerotic risk assessment.

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Year:  1988        PMID： 2967073     DOI： 10.1161/01.atv.8.3.212

Source DB:  PubMed          Journal:  Arteriosclerosis        ISSN： 0276-5047


  16 in total

1.  Expressed hypervariable polymorphism of apolipoprotein (a).

Authors:  M I Kamboh; R E Ferrell; B A Kottke
Journal:  Am J Hum Genet       Date:  1991-11       Impact factor: 11.025

2.  Reduction of Lp(a) by different methods of plasma exchange.

Authors:  I Schenck; C Keller; S Hailer; G Wolfram; N Zöllner
Journal:  Klin Wochenschr       Date:  1988-12-15

3.  A unique pattern of apo(a) polymorphism in an isolated east Greenlandic Inuit (Eskimo) population.

Authors:  I C Klausen; P S Hansen; J V Povlsen; L U Gerdes; L Lemming; O Faergeman
Journal:  Eur J Epidemiol       Date:  1995-10       Impact factor: 8.082

4.  Differences in apolipoprotein (a) polymorphism in west Greenland Eskimos and Caucasian Danes.

Authors:  I C Klausen; L U Gerdes; E B Schmidt; J Dyerberg; O Faergeman
Journal:  Hum Genet       Date:  1992-06       Impact factor: 4.132

5.  Cys4057 of apolipoprotein(a) is essential for lipoprotein(a) assembly.

Authors:  C Brunner; H G Kraft; G Utermann; H J Müller
Journal:  Proc Natl Acad Sci U S A       Date:  1993-12-15       Impact factor: 11.205

6.  A PvuII polymorphism of the low density lipoprotein receptor gene is not associated with plasma concentrations of low density lipoproteins including LP(a).

Authors:  I C Klausen; P S Hansen; L U Gerdes; N Rüdiger; N Gregersen; O Faergeman
Journal:  Hum Genet       Date:  1993-03       Impact factor: 4.132

7.  Hormonal regulation of serum Lp (a) levels. Opposite effects after estrogen treatment and orchidectomy in males with prostatic carcinoma.

Authors:  P Henriksson; B Angelin; L Berglund
Journal:  J Clin Invest       Date:  1992-04       Impact factor: 14.808

8.  Elevated plasma concentrations of lipoprotein(a) in patients with end-stage renal disease are not related to the size polymorphism of apolipoprotein(a).

Authors:  H Dieplinger; C Lackner; F Kronenberg; C Sandholzer; K Lhotta; F Hoppichler; H Graf; P König
Journal:  J Clin Invest       Date:  1993-02       Impact factor: 14.808

9.  Apolipoprotein E phenotype and lipoprotein(a) in familial hypercholesterolaemia: implication for lipoprotein(a) metabolism.

Authors:  G Lindahl; F Mailly; S Humphries; M Seed
Journal:  Clin Investig       Date:  1994-08

10.  Variation in the size of human apolipoprotein(a) is due to a hypervariable region in the gene.

Authors:  G Lindahl; E Gersdorf; H J Menzel; M Seed; S Humphries; G Utermann
Journal:  Hum Genet       Date:  1990-05       Impact factor: 4.132
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