Fabiana Almeida Curylofo-Zotti1, Muna S Elburki2, Priscilla Aparecida Oliveira3, Paulo Sérgio Cerri3, Leandro Alves Santos1, Hsi-Ming Lee2, Francis Johnson4, Lorne M Golub2, Carlos Rossa1, Morgana Rodrigues Guimarães-Stabili5. 1. Department of Restorative Dentistry School of Dentistry of Riberão Preto University of São Paulo Riberão Preto SP Brazil. 2. Department of Oral Biology and Pathology, School of Dental Medicine, SUNY at Stony Brook, Stony Brook, USA. 3. Laboratory of Histology and Embryology, School of Dentistry at Araraquara, UNESP, Araraquara, SP, Brazil. 4. Department of Chemistry and Pharmacological Sciences, SUNY at Stony Brook, Stony Brook, USA. 5. Department of Restorative Dentistry School of Dentistry of Riberão Preto University of São Paulo Riberão Preto SP Brazil. Electronic address: morganaguimaraes@foar.unesp.br.
Abstract
OBJECTIVE: The purpose of this study was to compare the effects of the oral administration of natural curcumin and a chemically modified curcumin (CMC2.24) on osteoclast-mediated bone resorption, apoptosis, and inflammation in a murine model of experimental periodontal disease. DESIGN: Fifty male rats were distributed among the following treatment groups: (i) 2% carboxymethylcellulose, (ii) CMC2.24 30 mg/kg body weight, (iii) Curcumin 100 mg/kg body weight and (iv) no treatment. Compounds were administered daily by oral intubation over a 15-day period of time. Periodontal disease was induced by injections of LPS (lipopolysaccharide) into the gingival tissues three times per week. Contralateral sides were injected with the same volume of PBS (phosphate buffered saline) vehicle. After 15 days, hemimaxillae and gingival tissues were harvested. Bone resorption was assessed by μCT (microcomputer tomography). Formalin-fixed, paraffin embedded histological sections were stained with haematoxylin/eosin (H/E) for the assessment of cellular infiltrate or subjected to immunohistochemistry for detecting TRAP (tartrate-resistant acid phosphatase)-positive cells and caspase-3. Apoptosis was assessed in the gingival tissues by DNA fragmentation. RESULTS: CMC2.24 and curcumin caused a significant reduction of the inflammatory cell infiltrate, however μCT analysis showed that only CMC2.24 reduced bone resorption and the number of TRAP-positive multinucleated cells (osteoclasts). Curcumin, but not CMC2.24, significantly reduced the number of apoptotic cells in the gingival tissues and of osteocytes in the alveolar bone crest. CONCLUSIONS: The results suggest that CMC2.24 and curcumin inhibit inflammation by different mechanisms, but only CMC2.24 was capable of reducing alveolar bone resorption in the LPS-induced model of periodontitis.
OBJECTIVE: The purpose of this study was to compare the effects of the oral administration of natural curcumin and a chemically modified curcumin (CMC2.24) on osteoclast-mediated bone resorption, apoptosis, and inflammation in a murine model of experimental periodontal disease. DESIGN: Fifty male rats were distributed among the following treatment groups: (i) 2% carboxymethylcellulose, (ii) CMC2.24 30 mg/kg body weight, (iii) Curcumin 100 mg/kg body weight and (iv) no treatment. Compounds were administered daily by oral intubation over a 15-day period of time. Periodontal disease was induced by injections of LPS (lipopolysaccharide) into the gingival tissues three times per week. Contralateral sides were injected with the same volume of PBS (phosphate buffered saline) vehicle. After 15 days, hemimaxillae and gingival tissues were harvested. Bone resorption was assessed by μCT (microcomputer tomography). Formalin-fixed, paraffin embedded histological sections were stained with haematoxylin/eosin (H/E) for the assessment of cellular infiltrate or subjected to immunohistochemistry for detecting TRAP (tartrate-resistant acid phosphatase)-positive cells and caspase-3. Apoptosis was assessed in the gingival tissues by DNA fragmentation. RESULTS:CMC2.24 and curcumin caused a significant reduction of the inflammatory cell infiltrate, however μCT analysis showed that only CMC2.24 reduced bone resorption and the number of TRAP-positive multinucleated cells (osteoclasts). Curcumin, but not CMC2.24, significantly reduced the number of apoptotic cells in the gingival tissues and of osteocytes in the alveolar bone crest. CONCLUSIONS: The results suggest that CMC2.24 and curcumin inhibit inflammation by different mechanisms, but only CMC2.24 was capable of reducing alveolar bone resorption in the LPS-induced model of periodontitis.
Authors: Morgana R Guimaraes-Stabili; Marcell Costa de Medeiros; Danuza Rossi; Angelo Constantino Camilli; Cleslei Fernando Zanelli; Sandro Roberto Valentini; Luis Carlos Spolidorio; Keith Lough Kirkwood; Carlos Rossa Journal: Clin Oral Investig Date: 2020-11-02 Impact factor: 3.573