Merdan Serin1, Dincer Altinel1, Cem Leblebici2, Burcu Biltekin3, Mert Celikten4, Fatih Irmak5, Sevgi Kurt Yazar1. 1. Department of Plastic and Reconstructive Surgery, Istanbul Training and Research Hospital, Istanbul, Turkey. 2. Department of Pathology, Istanbul Training and Research Hospital, Istanbul, Turkey. 3. Department of Histology and Embryology, Cerrahpasa Medical School, Istanbul University, Istanbul, Turkey. 4. Animal Research Laboratory, Medical School, Istanbul Bezmialem University, Istanbul, Turkey. 5. Department of Plastic and Reconstructive Surgery, Sisli Etfal Training and Research Hospital, Istanbul, Turkey.
Abstract
PURPOSE: To investigate the effect of subcutaneous sildenafil on random flap survival. METHODS: Fourteen Wistar rats, which were divided in to two groups, were used for this experimental study. Rats in the sildenafil group received subcutaneous sildenafil injections daily for seven days before flap elevation. At the end of the treatment period, 9x3 cm dorsal skin flaps were elevated and reinserted back into their place in all of the animals. Necrotic and whole flaps areas were recorded on graph papers. Seven days after the flap elevation samples for histological examination were taken and angiographies were performed to visualize the flap vascularization. RESULTS: The calculated average percentage of necrotic flap areas were 18.29% and 42.26% in the sildenafil and control group respectively.(p=0.0233). In selected angiography images, vessels were found to be more prominent in the sildenafil group. The average number of capillary formations under light microscopy was higher in the sildenafil group (p= 0.0286). CONCLUSION: The subdermal high dose sildenafil has a positive effect on flap survival.
PURPOSE: To investigate the effect of subcutaneous sildenafil on random flap survival. METHODS: Fourteen Wistar rats, which were divided in to two groups, were used for this experimental study. Rats in the sildenafil group received subcutaneous sildenafil injections daily for seven days before flap elevation. At the end of the treatment period, 9x3 cm dorsal skin flaps were elevated and reinserted back into their place in all of the animals. Necrotic and whole flaps areas were recorded on graph papers. Seven days after the flap elevation samples for histological examination were taken and angiographies were performed to visualize the flap vascularization. RESULTS: The calculated average percentage of necrotic flap areas were 18.29% and 42.26% in the sildenafil and control group respectively.(p=0.0233). In selected angiography images, vessels were found to be more prominent in the sildenafil group. The average number of capillary formations under light microscopy was higher in the sildenafil group (p= 0.0286). CONCLUSION: The subdermal high dose sildenafil has a positive effect on flap survival.
Authors: Mohamed A Ellabban; Islam Omar Abdel Fattah; Ghada Abdel Kader; Omar Salah Eldeen; Amir E Mehana; Dina M Khodeer; Hossam Hosny; Mahmoud S Elbasiouny; Suhail Masadeh Journal: Sci Rep Date: 2020-02-21 Impact factor: 4.379
Authors: Rafael A C Souza; Carla Patrícia Martinelli-Kläy; Armando J d'Acampora; Geraldo J S Bernardes; Sandro M Sgrott; Laila A C Souza; Tommaso Lombardi; Thaís R Sudbrack Journal: Arch Dermatol Res Date: 2021-03-14 Impact factor: 3.017