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Literature DB >> 29663108

TROSY pulse sequence for simultaneous measurement of the ¹⁵N R₁ and {¹H}-¹⁵N NOE in deuterated proteins.

Abstract

A TROSY-based NMR experiment is described for simultaneous measurement of the 15N longitudinal relaxation rate constant R1 and the {1H}-15N nuclear Overhauser enhancement. The experiment is based on the observation that the TROSY mixing pulse sequence element symmetrically exchanges 1H and 15N magnetizations. The accuracy of the proposed technique is validated by comparison to independent measurements of both relaxation parameters for the protein ubiquitin. The simultaneous experiment is approximately 20-33% shorter than conventional sequential measurements.

Entities: CellLine Chemical Disease Species

Keywords: Dynamics; Longitudinal relaxation; Nuclear Overhauser enhancement; Protein; Spin–lattice relaxation; TROSY

Mesh：

Substances：

Year: 2018 PMID： 29663108 PMCID： PMC6510663 DOI： 10.1007/s10858-018-0181-6

Source DB: PubMed Journal: J Biomol NMR ISSN： 0925-2738 Impact factor: 2.835

11 in total

1. Protein dynamics measurements by TROSY-based NMR experiments.

Authors: G Zhu; Y Xia; L K Nicholson; K H Sze
Journal: J Magn Reson Date: 2000-04 Impact factor: 2.229

2. NMR characterization of the dynamics of biomacromolecules.

Authors: Arthur G Palmer
Journal: Chem Rev Date: 2004-08 Impact factor: 60.622

3. Recovering lost magnetization: polarization enhancement in biomolecular NMR.

Authors: Adrien Favier; Bernhard Brutscher
Journal: J Biomol NMR Date: 2010-12-30 Impact factor: 2.835

4. A 1H-NMR thermometer suitable for cryoprobes.

Authors: M Findeisen; T Brand; S Berger
Journal: Magn Reson Chem Date: 2007-02 Impact factor: 2.447

5. NMRPipe: a multidimensional spectral processing system based on UNIX pipes.

Authors: F Delaglio; S Grzesiek; G W Vuister; G Zhu; J Pfeifer; A Bax
Journal: J Biomol NMR Date: 1995-11 Impact factor: 2.835

6. Measurement of ¹⁵N relaxation rates in perdeuterated proteins by TROSY-based methods.

Authors: Nils-Alexander Lakomek; Jinfa Ying; Ad Bax
Journal: J Biomol NMR Date: 2012-06-12 Impact factor: 2.835

7. Accurate sampling of high-frequency motions in proteins by steady-state (15)N-{(1)H} nuclear Overhauser effect measurements in the presence of cross-correlated relaxation.

Authors: Fabien Ferrage; David Cowburn; Ranajeet Ghose
Journal: J Am Chem Soc Date: 2009-05-06 Impact factor: 15.419

TROSY pulse sequence for simultaneous measurement of the ¹⁵N R₁ and {¹H}-¹⁵N NOE in deuterated proteins.

1. Protein dynamics measurements by TROSY-based NMR experiments.

2. NMR characterization of the dynamics of biomacromolecules.

3. Recovering lost magnetization: polarization enhancement in biomolecular NMR.

4. A 1H-NMR thermometer suitable for cryoprobes.

5. NMRPipe: a multidimensional spectral processing system based on UNIX pipes.

6. Measurement of ¹⁵N relaxation rates in perdeuterated proteins by TROSY-based methods.

7. Accurate sampling of high-frequency motions in proteins by steady-state (15)N-{(1)H} nuclear Overhauser effect measurements in the presence of cross-correlated relaxation.

8. Frequency spectrum of NH bonds in eglin c from spectral density mapping at multiple fields.

9. Dynamics of GCN4 facilitate DNA interaction: a model-free analysis of an intrinsically disordered region.

10. An optimized method for (15)N R(1) relaxation rate measurements in non-deuterated proteins.

1. Dynamic ¹⁵N{¹H} NOE measurements: a tool for studying protein dynamics.