Maria Apostolopoulou1,2,3, Barbara Menart-Houtermans2,3, Ruth Ruetter2,3, Bettina Nowotny2,3, Ulrich Gehrmann3,4, Daniel Markgraf2,3, Julia Szendroedi1,2,3, Nanette C Schloot2, Michael Roden5,6,7. 1. Division of Endocrinology and Diabetology, Medical Faculty, c/o German Diabetes Center, Heinrich-Heine University, Düsseldorf, Auf dem Hennekamp 65, 40225, Düsseldorf, Germany. 2. Institute for Clinical Diabetology, German Diabetes Center, Leibniz Center for Diabetes Research at Heinrich-Heine University Düsseldorf, Düsseldorf, Germany. 3. German Center for Diabetes Research, Düsseldorf, Germany. 4. Institute for Biometrics and Epidemiology, German Diabetes Center, Leibniz Center for Diabetes Research at Heinrich-Heine University, Düsseldorf, Germany. 5. Division of Endocrinology and Diabetology, Medical Faculty, c/o German Diabetes Center, Heinrich-Heine University, Düsseldorf, Auf dem Hennekamp 65, 40225, Düsseldorf, Germany. michael.roden@ddz.uni-duesseldorf.de. 6. Institute for Clinical Diabetology, German Diabetes Center, Leibniz Center for Diabetes Research at Heinrich-Heine University Düsseldorf, Düsseldorf, Germany. michael.roden@ddz.uni-duesseldorf.de. 7. German Center for Diabetes Research, Düsseldorf, Germany. michael.roden@ddz.uni-duesseldorf.de.
Abstract
AIMS: Infiltration of pancreatic islets with different leukocyte subtypes likely contributes to deterioration of glycemia in diabetes mellitus. Different subsets of leukocytes have been previously associated with type 1 or type 2 diabetes. This study aimed at examining these subsets at different stages of diabetes progression and possible relationships with metabolic parameters. METHODS: A total of 206 patients, 76 with type 1 and 130 with type 2 diabetes, were studied within the first year of diabetes diagnosis. In addition, 31 patients with type 1 and 73 with type 2 diabetes were examined at 5 years after diagnosis. Whole body insulin sensitivity was assessed by hyperinsulinemic-euglycemic clamps; insulin secretion by glucagon stimulation tests and white blood cells were analyzed by flow cytometry. RESULTS: The percentage of peripheral CD8+ cells was 15% lower in patients with type 1 diabetes at 5 years than in patients at diabetes onset and correlated positively with fasting glycemia, total cholesterol and high-sensitive C-reactive protein (hsCRP) (all r > 0.37, p < 0.05), but not with insulin secretion. Patients with type 2 diabetes had 7% higher percentages of CD4+ cells after 5 years than those at diagnosis. CD4+ cells correlated with hsCRP (r = 0.36, p < 0.05), whereas CD8+ cytotoxic T-cells did not correlate with any metabolic parameter. CONCLUSION: CD8+ T-cells associate with worse glycemia, lipidemia and inflammation after 5 years of type 1 diabetes, whereas CD4+ T-cells associate with increased inflammation after 5 years upon onset of type 2 diabetes.
AIMS: Infiltration of pancreatic islets with different leukocyte subtypes likely contributes to deterioration of glycemia in diabetes mellitus. Different subsets of leukocytes have been previously associated with type 1 or type 2 diabetes. This study aimed at examining these subsets at different stages of diabetes progression and possible relationships with metabolic parameters. METHODS: A total of 206 patients, 76 with type 1 and 130 with type 2 diabetes, were studied within the first year of diabetes diagnosis. In addition, 31 patients with type 1 and 73 with type 2 diabetes were examined at 5 years after diagnosis. Whole body insulin sensitivity was assessed by hyperinsulinemic-euglycemic clamps; insulin secretion by glucagon stimulation tests and white blood cells were analyzed by flow cytometry. RESULTS: The percentage of peripheral CD8+ cells was 15% lower in patients with type 1 diabetes at 5 years than in patients at diabetes onset and correlated positively with fasting glycemia, total cholesterol and high-sensitive C-reactive protein (hsCRP) (all r > 0.37, p < 0.05), but not with insulin secretion. Patients with type 2 diabetes had 7% higher percentages of CD4+ cells after 5 years than those at diagnosis. CD4+ cells correlated with hsCRP (r = 0.36, p < 0.05), whereas CD8+ cytotoxic T-cells did not correlate with any metabolic parameter. CONCLUSION: CD8+ T-cells associate with worse glycemia, lipidemia and inflammation after 5 years of type 1 diabetes, whereas CD4+ T-cells associate with increased inflammation after 5 years upon onset of type 2 diabetes.
Authors: Nels C Olson; Margaret F Doyle; Colleen M Sitlani; Ian H de Boer; Stephen S Rich; Sally A Huber; Alan L Landay; Russell P Tracy; Bruce M Psaty; Joseph A Delaney Journal: J Clin Endocrinol Metab Date: 2020-03-01 Impact factor: 5.958
Authors: Tiffany R Butterfield; David B Hanna; Robert C Kaplan; Xiaonan Xue; Jorge R Kizer; Helen G Durkin; Seble G Kassaye; Marek Nowicki; Phyllis C Tien; Elizabeth T Topper; Michelle A Floris-Moore; Kehmia Titanji; Margaret A Fischl; Sonya Heath; Clovis S Palmer; Alan L Landay; Joshua J Anzinger Journal: AIDS Date: 2022-06-21 Impact factor: 4.632
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