| Literature DB >> 29662172 |
Mario Brameshuber1, Florian Kellner2, Benedikt K Rossboth3, Haisen Ta4, Kevin Alge2, Eva Sevcsik3, Janett Göhring3,2, Markus Axmann5, Florian Baumgart3, Nicholas R J Gascoigne6, Simon J Davis7, Hannes Stockinger2, Gerhard J Schütz3, Johannes B Huppa8.
Abstract
T cell antigen recognition requires T cell antigen receptors (TCRs) engaging MHC-embedded antigenic peptides (pMHCs) within the contact region of a T cell with its conjugated antigen-presenting cell. Despite micromolar TCR:pMHC affinities, T cells respond to even a single antigenic pMHC, and higher-order TCRs have been postulated to maintain high antigen sensitivity and trigger signaling. We interrogated the stoichiometry of TCRs and their associated CD3 subunits on the surface of living T cells through single-molecule brightness and single-molecule coincidence analysis, photon-antibunching-based fluorescence correlation spectroscopy and Förster resonance energy transfer measurements. We found exclusively monomeric TCR-CD3 complexes driving the recognition of antigenic pMHCs, which underscores the exceptional capacity of single TCR-CD3 complexes to elicit robust intracellular signaling.Entities:
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Year: 2018 PMID： 29662172 PMCID： PMC7612939 DOI： 10.1038/s41590-018-0092-4
Source DB: PubMed Journal: Nat Immunol ISSN： 1529-2908 Impact factor: 31.250