| Literature DB >> 29661317 |
Yi Yang1, Xiaoliang Zhao1, Jia Li1, Hao Jiang1, Xindi Shan1, Ya Wang1, Wenbang Ma1, Jiejie Hao2, Guangli Yu3.
Abstract
We studied the mechanisms underlying the immunostimulatory effects of aβ-1,3/1,6-glucan (BG136) from Durvillaea Antarctica. Our data showed that BG136 promoted the activation of MAPKs and NF-κB signaling pathways and cytokines production. BG136 did not increase MCP-1 or NO production or phosphorylation of NF-κB and MAPK in TLR4 siRNA knockdown cells, indicating that BG136 activates macrophages through TLR4. Flow cytometry analysis and confocal experiment showed that BG136 bound to TLR4 expressed on RAW264.7 macrophage cells surface. The affinity of BG136 for TLR4 was determined using Surface Plasmon Resonance (SPR) (KD: 4.51 × 10-6M). Altogether, our results showed that BG136 activates RAW264.7 cells by binding to TLR4 and then triggering TLR4-mediated signaling pathways to promote cytokines secretion.Entities:
Keywords: Immunomodulatory effect; MAPK pathway; NF-κB; RAW264.7 macrophage; TLR4; β-glucan
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Year: 2018 PMID： 29661317 DOI： 10.1016/j.carbpol.2018.03.019
Source DB: PubMed Journal: Carbohydr Polym ISSN： 0144-8617 Impact factor: 9.381