Literature DB >> 2966074

Site directed mutants of human interleukin-1 alpha: a 1H-NMR and receptor binding study.

A M Gronenborn1, P T Wingfield, H R McDonald, U Schmeissner, G M Clore.   

Abstract

Mutant human interleukin-1 alpha proteins were constructed by oligonucleotide directed mutagenesis. Six different mutants were tested for receptor binding activity and showed no alteration with respect to the wild-type protein. Analysis of these mutants by nuclear magnetic resonance spectroscopy confirmed the structural integrity of the mutant proteins and permitted the sequence specific assignment of the histidine and tryptophan residues.

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Year:  1988        PMID： 2966074     DOI： 10.1016/0014-5793(88)80717-8

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  3 in total

1.  Crystallographic refinement of interleukin 1 beta at 2.0 A resolution.

Authors:  J P Priestle; H P Schär; M G Grütter
Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205

2.  Analysis of mutations in the putative nuclear localization sequence of interleukin-1 beta.

Authors:  S Grenfell; N Smithers; S Witham; A Shaw; P Graber; R Solari
Journal:  Biochem J       Date:  1991-11-15       Impact factor: 3.857

3.  Identification of the discontinuous binding site in human interleukin 1 beta for the type I interleukin 1 receptor.

Authors:  E Labriola-Tompkins; C Chandran; K L Kaffka; D Biondi; B J Graves; M Hatada; V S Madison; J Karas; P L Kilian; G Ju
Journal:  Proc Natl Acad Sci U S A       Date:  1991-12-15       Impact factor: 11.205
  3 in total

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