| Literature DB >> 29656242 |
Yue Wang1, Fengge Zhai2, Yasushi Hasebe3, Hongmin Jia2, Zhiqiang Zhang4.
Abstract
The fabrication, characterization and analytical performance were investigated for a phenol biosensor based on the covalent bonding of tyrosinase (TYR) onto a graphene oxide (GO)-modified glassy carbon electrode (GCE) via glutaraldehyde (GA). The surface morphology of the modified electrode was studied by atomic force microscope (AFM) and field-emission scanning electron microscopy (FE-SEM). The fabricated TYR/GA/GO/GCE biosensor showed very good stability, reproducibility, sensitivity and practical usage. The catechol biosensor exhibited a wide sensing linear range from 5×10-8M to 5×10-5M, a lower detection limit of 3×10-8M, a current maximum (Imax) of 65.8μA and an apparent Michaelis constant (Kmapp) of 169.9μM.Entities:
Keywords: Covalent bonding; Glassy carbon electrode; Graphene oxide; Phenol derivatives; Tyrosinase
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Year: 2018 PMID: 29656242 DOI: 10.1016/j.bioelechem.2018.04.003
Source DB: PubMed Journal: Bioelectrochemistry ISSN: 1567-5394 Impact factor: 5.373