Graciela Del Valle Castillo1, Silvia López de Blanc2, Claudia Elena Sotomayor3, Ana Isabel Azcurra4. 1. Dpto. de Biología Bucal, Facultad de Odontología, Universidad Nacional de Córdoba, Argentina. Electronic address: graciela.castillo@unc.edu.ar. 2. Dpto. de Patología Bucal, Facultad de Odontología, Universidad Nacional de Córdoba, Argentina. 3. Dpto. de Bioquímica Clínica- CIBICI-CONICET, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina. 4. Dpto. de Biología Bucal, Facultad de Odontología, Universidad Nacional de Córdoba, Argentina.
Abstract
OBJECTIVE: The aim of this study was to explore the association between malignant and premalignant lesions and the virulence factor profile of Candida spp. recovered from different oral lesions. DESIGN: Candida spp. isolated from malignant lesions (squamous cell carcinoma, OC, n = 25), atypical lichen planus (AL, n = 11), chronic candidiasis (CC, n = 25), and asymptomatic carriers (WI, n = 15, control strains.) Isolates were identified in chromogenic medium, colony morphology and biochemical tests. The lipolytic and proteinase activity was determined on supplemented agar with olive oil and BSA, respectively. The biofilm formation with XTT reduction assay and cellular surface hydrophobicity (CSH) by water-hydrocarbon method were performed. RESULTS: All isolates recovered from oral lesions produced the four virulence factors studied with significantly higher levels than in WI isolates. Interestingly, lipolytic activity was absent in WI isolates. The proteolytic activity was similar in AL and OC isolates. OC isolates showed significantly higher CSH values than other clinical isolates. Non-albicans species showed higher biofilm formation than C.albicans (P = 0.03.) There were no significant differences in virulence factors among species. A strong positive correlation was found between proteinase and lipase activity (r = 0.90, P < 0.0001), and between hydrophobicity and biofilm (R = 0.81, P < 0.0001.) CONCLUSIONS: Our results indicate that OC Candida isolates exhibited a significant higher attributes of virulence than other lesions fungus isolates, providing evidence about the association between Candida pathogenicity and lesions severity.
OBJECTIVE: The aim of this study was to explore the association between malignant and premalignant lesions and the virulence factor profile of Candida spp. recovered from different oral lesions. DESIGN: Candida spp. isolated from malignant lesions (squamous cell carcinoma, OC, n = 25), atypical lichen planus (AL, n = 11), chronic candidiasis (CC, n = 25), and asymptomatic carriers (WI, n = 15, control strains.) Isolates were identified in chromogenic medium, colony morphology and biochemical tests. The lipolytic and proteinase activity was determined on supplemented agar with olive oil and BSA, respectively. The biofilm formation with XTT reduction assay and cellular surface hydrophobicity (CSH) by water-hydrocarbon method were performed. RESULTS: All isolates recovered from oral lesions produced the four virulence factors studied with significantly higher levels than in WI isolates. Interestingly, lipolytic activity was absent in WI isolates. The proteolytic activity was similar in AL and OC isolates. OC isolates showed significantly higher CSH values than other clinical isolates. Non-albicans species showed higher biofilm formation than C.albicans (P = 0.03.) There were no significant differences in virulence factors among species. A strong positive correlation was found between proteinase and lipase activity (r = 0.90, P < 0.0001), and between hydrophobicity and biofilm (R = 0.81, P < 0.0001.) CONCLUSIONS: Our results indicate that OC Candida isolates exhibited a significant higher attributes of virulence than other lesions fungus isolates, providing evidence about the association between Candida pathogenicity and lesions severity.
Authors: Susiane S Moreira-Oliveira; Lucas Amaral-Machado; Wógenes Nunes de Oliveira; Éverton N Alencar; Kelly Cristine Zatta; Luanda B F C de Souza; Aldo da Cunha Medeiros; Guilherme Maranhão Chaves; Eryvaldo S T Egito Journal: Pharmaceutics Date: 2018-12-03 Impact factor: 6.321
Authors: Priyanka Debta; Santosh Kumar Swain; Mahesh Chandra Sahu; Abdulwahab A Abuderman; Khalid J Alzahrani; Hamsa Jameel Banjer; Ahtesham Ahmad Qureshi; Mohammed Mousa H Bakri; Gargi S Sarode; Sangram Patro; Saswati Siddhartha; Shankargouda Patil Journal: Int J Environ Res Public Health Date: 2022-07-12 Impact factor: 4.614
Authors: María Soledad Miró; Juan Pablo Caeiro; Emilse Rodriguez; Lara Vargas; Cecilia Vigezzi; Paula A Icely; Graciela D V Castillo; Ana I Azcurra; Claudio D Abiega; Fernando O Riera; Claudia E Sotomayor Journal: J Fungi (Basel) Date: 2021-12-28