Francisco Samuel Rodrigues Carvalho1, Victor Pinheiro Feitosa2, Paulo Goberlânio de Barros Silva3, Eduardo Costa Studart Soares4, Thyciana Rodrigues Ribeiro5, Cristiane Sá Roriz Fonteles5, Fábio Wildson Gurgel Costa6. 1. Division of Oral and Maxillofacial Surgery, Federal University of Ceará, Fortaleza, Brazil; Division of Oral and Maxillofacial Morphology and Oral and Maxillofacial Surgery, UNIFOR, Fortaleza, Brazil. Electronic address: samuelcarvalho@unifor.br. 2. Paulo Picanço School of Dentistry, Fortaleza, Ceará, Brazil. 3. Unichristus University Center, Fortaleza, Ceará, Brazil. 4. Division of Oral and Maxillofacial Surgery, Walter Cantídio University Hospital, Federal University of Ceará, Fortaleza, Brazil. 5. School of Dentistry, Federal University of Ceará, Ceará, Fortaleza, Brazil. 6. Division of Oral Radiology, Walter Cantídio University Hospital, Federal University of Ceará, Fortaleza, Brazil.
Abstract
PURPOSE: To evaluate different therapeutic Carnoy's solution formulations on hard human tissues. MATERIALS AND METHODS: An in vitro study was performed with human teeth (n = 36) and bone fragments (n = 18), randomly divided into two experimental groups (Group I = Carnoy solution with chloroform; Group II = Carnoy solution without chloroform) and a control group (saline solution). The groups were subdivided into pre-conditioning, post-conditioning, and post-conditioning with saline washing. Raman microspectroscopy, Knoop microhardness test, and scanning electron microscopy with energy dispersive X-ray spectroscopy were used. RESULTS: There was demineralization of dental structures regarding mineral/matrix and carbonate/phosphate ratios (GI versus GII, p < 0.05). The presence of chloroform resulted in a statistically significant reduction of the teeth surface microhardness (p = 0.036), but not exceeding 0.01 μm. Both GI and GII showed significant structural changes by using scanning electron microscopy with energy dispersive X-ray spectroscopy. CONCLUSION: Carnoy's solution altered the organic and inorganic matrix of the human calcified specimens analyzed in vitro, and its effect was more pronounced when chloroform was present.
PURPOSE: To evaluate different therapeutic Carnoy's solution formulations on hard human tissues. MATERIALS AND METHODS: An in vitro study was performed with human teeth (n = 36) and bone fragments (n = 18), randomly divided into two experimental groups (Group I = Carnoy solution with chloroform; Group II = Carnoy solution without chloroform) and a control group (saline solution). The groups were subdivided into pre-conditioning, post-conditioning, and post-conditioning with saline washing. Raman microspectroscopy, Knoop microhardness test, and scanning electron microscopy with energy dispersive X-ray spectroscopy were used. RESULTS: There was demineralization of dental structures regarding mineral/matrix and carbonate/phosphate ratios (GI versus GII, p < 0.05). The presence of chloroform resulted in a statistically significant reduction of the teeth surface microhardness (p = 0.036), but not exceeding 0.01 μm. Both GI and GII showed significant structural changes by using scanning electron microscopy with energy dispersive X-ray spectroscopy. CONCLUSION: Carnoy's solution altered the organic and inorganic matrix of the human calcified specimens analyzed in vitro, and its effect was more pronounced when chloroform was present.
Authors: Francisco Samuel Rodrigues Carvalho; Marina Mota Lima Verde; Khalil Fernandes Viana; Thâmara Manoela Marinho Bezerra; Said Gonçalves da Cruz Fonseca; Karuza Maria Alves Pereira; Thyciana Rodrigues Ribeiro; Fábio Wildson Gurgel Costa Journal: Asian Pac J Cancer Prev Date: 2019-11-01