The threshold of influent ammonium concentration for nitrate over-accumulation in a one-stage deammonification system with granular sludge without aeration.

Low-strength ammonium is still a challenge for the mainstream deammonification because of nitrate over-accumulation. In this study, the threshold of influent ammonium concentration of one-stage deammonification system with granular sludge was investigated, by stepwise decreasing influent ammonium from high concentrations (280mg/L to 140mg/L) to the low concentration (70mg/L) in 108d at 32°C without aeration. Results showed that, under 70mg/L NH4+-N, ΔNO3--N/ΔNH4+-N ratio increased to 0.2, deviated from the theoretical value of 0.11, with ammonium and TN removal efficiencies of 91% and 71%, respectively. However, under both high ammonium concentrations (280mg/L and 140mg/L), nitrate production stabilized at only 13%. Chloroflexi, Planctomycetes and Proteobacteria contributed >70% of the communities under all three ammonium concentrations. As influent ammonium decreasing, the relative abundances of bacteria for anammox, aerobic oxidizing and denitrifying decreased, while NOB (nitrite oxidizing bacteria) abundance increased greatly. So 70mg/L was the threshold of influent ammonium concentration for stable deammonification without organic influent. It was the decrease of functional bacteria and overgrowth of NOB that worsen the deammonification performance under low-strength ammonium.