Letícia Helena Theodoro1, Naida Zanini Assem2, Mariéllen Longo3, Márcio Luiz Ferro Alves4, Cristiane Duque5, Rafael Nobrega Stipp6, Natália Leal Vizoto7, Valdir Gouveia Garcia8. 1. Department of Surgery and Integrated Clinic, São Paulo State University (UNESP), Dentistry School of Araçatuba, Brazil. Electronic address: letheodoro@foa.unesp.br. 2. Department of Surgery and Integrated Clinic, São Paulo State University (UNESP), Dentistry School of Araçatuba, Brazil. Electronic address: naidaassem@uol.com.br. 3. Department of Surgery and Integrated Clinic, São Paulo State University (UNESP), Dentistry School of Araçatuba, Brazil. Electronic address: mary.lonngo@hotmail.com. 4. Department of Surgery and Integrated Clinic, São Paulo State University (UNESP), Dentistry School of Araçatuba, Brazil. Electronic address: marcimferro@gmail.com. 5. Department of Pediatric and Social Dentistry, São Paulo State University (UNESP), Dentistry School of Araçatuba, Brazil. Electronic address: cduque@foa.unesp.br. 6. Department of Oral Diagnosis, Dentistry School of Piracicaba, UNICAMP, Brazil. Electronic address: rafaelns@fop.unicamp.br. 7. Department of Oral Diagnosis, Dentistry School of Piracicaba, UNICAMP, Brazil. Electronic address: biomed.natalia@gmail.com. 8. Department of Surgery and Integrated Clinic, São Paulo State University (UNESP), Dentistry School of Araçatuba, Brazil. Electronic address: vgouveia@foa.unesp.br.
Abstract
BACKGROUND: The aim of this study was to evaluate the effects of non-surgical periodontal therapies on smokers with chronic periodontitis, involving multiple adjunctive applications of antimicrobial photodynamic therapy (aPDT), and systemic metronidazole (MTZ) with amoxicillin (AMX). METHODS: All participants were treated with scaling and root planing (SRP). Seventeen patients received 400 mg of MTZ and 500 mg of AMX three times per day for 7 days (MTZ + AMX). Additionally, 17 patients received a placebo, and 17 patients were treated with three applications of aPDT (immediately, 48 h and 96 h after SRP). Clinical and microbiological examinations were performed at baseline and at 90 and 180 days post-therapy. Subgingival samples were analyzed using real-time polymerase chain reaction. RESULTS: After 180 days, the patients in groups MTZ + AMX and aPDT had significantly lower mean probing depths, more clinical attachment level gains and less bleeding on probing. At 180 days, in the moderate pocket there was a reduction in the levels of Porphyromonas gingivalis and Prevotella nigrescens in the MTZ + AMX group, while group aPDT showed a reduction in Prevotella nigrescens. Furthermore, at 180 days, in the deep pocket a reduction in Porphyromonas gingivalis, Prevotella intermedia and Prevotella nigrescens was observed in group MTZ + AMX, as well as a reduction in the levels of Prevotella intermedia and Prevotella nigrescens in group aPDT. CONCLUSION: In smokers with periodontitis, the MTZ + AMX and aPDT treatments significantly improved the effects of SRP.
RCT Entities:
BACKGROUND: The aim of this study was to evaluate the effects of non-surgical periodontal therapies on smokers with chronic periodontitis, involving multiple adjunctive applications of antimicrobial photodynamic therapy (aPDT), and systemic metronidazole (MTZ) with amoxicillin (AMX). METHODS: All participants were treated with scaling and root planing (SRP). Seventeen patients received 400 mg of MTZ and 500 mg of AMX three times per day for 7 days (MTZ + AMX). Additionally, 17 patients received a placebo, and 17 patients were treated with three applications of aPDT (immediately, 48 h and 96 h after SRP). Clinical and microbiological examinations were performed at baseline and at 90 and 180 days post-therapy. Subgingival samples were analyzed using real-time polymerase chain reaction. RESULTS: After 180 days, the patients in groups MTZ + AMX and aPDT had significantly lower mean probing depths, more clinical attachment level gains and less bleeding on probing. At 180 days, in the moderate pocket there was a reduction in the levels of Porphyromonas gingivalis and Prevotella nigrescens in the MTZ + AMX group, while group aPDT showed a reduction in Prevotella nigrescens. Furthermore, at 180 days, in the deep pocket a reduction in Porphyromonas gingivalis, Prevotella intermedia and Prevotella nigrescens was observed in group MTZ + AMX, as well as a reduction in the levels of Prevotella intermedia and Prevotella nigrescens in group aPDT. CONCLUSION: In smokers with periodontitis, the MTZ + AMX and aPDT treatments significantly improved the effects of SRP.
Authors: Aymeric Courval; Laetitia Harmouche; Anne Mathieu; Catherine Petit; Olivier Huck; François Séverac; Jean-Luc Davideau Journal: Int J Environ Res Public Health Date: 2020-06-11 Impact factor: 3.390