| Literature DB >> 29651746 |
Ali Jahanbazi Jahan-Abad1,2, Sajad Sahab Negah1,3, Hassan Hosseini Ravandi1, Sedigheh Ghasemi1, Maryam Borhani-Haghighi1, Walter Stummer4, Ali Gorji5,6,7,8,9, Maryam Khaleghi Ghadiri4.
Abstract
Traumatic brain injury (TBI) is a disruption in the brain functions following a head trauma. Cell therapy may provide a promising treatment for TBI. Among different cell types, human neural stem cells cultured in self-assembling peptide scaffolds have been suggested as a potential novel method for cell replacement treatment after TBI. In the present study, we accessed the effects of human neural stem/progenitor cells (hNS/PCs) derived from epileptic human brain and human adipose-derived stromal/stem cells (hADSCs) seeded in PuraMatrix hydrogel (PM) on brain function after TBI in an animal model of brain injury. hNS/PCs were isolated from patients with medically intractable epilepsy undergone epilepsy surgery. hNS/PCs and hADSCs have the potential for proliferation and differentiation into both neuronal and glial lineages. Assessment of the growth characteristics of hNS/PCs and hADSCs revealed that the hNS/PCs doubling time was significantly longer and the growth rate was lower than hADSCs. Transplantation of hNS/PCs and hADSCs seeded in PM improved functional recovery, decreased lesion volume, inhibited neuroinflammation, and reduced the reactive gliosis at the injury site. The data suggest the transplantation of hNS/PCs or hADSCs cultured in PM as a promising treatment option for cell replacement therapy in TBI.Entities:
Keywords: Epilepsy; Human neural stem cells; Inflammation; Tissue engineering; Traumatic brain injury
Mesh：
Substances：
Year: 2018 PMID： 29651746 DOI： 10.1007/s12035-018-1050-8
Source DB: PubMed Journal: Mol Neurobiol ISSN： 0893-7648 Impact factor: 5.590