BACKGROUND: Osteoarthritis (OA) is one of the complex diseases that affect a large population of the world. The aim of the current study was to explore the roles of taurine in OA rat models, and discover the related mechanisms. METHODS: OA rat models were established by anterior cruciate ligament transection (ACLT) plus medial meniscus resection (MMx) surgery on the right knees. Secondary mechanical allodynia, weight-bearing alterations and knee joint width were evaluated before surgery and every two weeks after surgery. At 14weeks, histopathological analysis was conducted on the knee joint cartilage. Protein amount of MMP-3 and CHOP was evaluated by western blot. RESULTS: Taurine injection after surgery significantly relieved the symptoms of OA in rat models in a dose-dependent and time-dependent manner, as shown by alleviation of secondary mechanical allodynia, decrease in hind limb weight-bearing alterations, and inhibited knee swelling. Moreover, histopathological analysis showed that taurine inhibited matrix loss and cartilage degeneration in a dose-dependent manner. Taurine administration strikingly suppressed the expression of matrix metalloproteinase-3 (MMP-3) and CHOP. CONCLUSION: These results indicated that taurine administration exhibited protective effects by inhibiting MMP-3 and CHOP expression, and subsequently alleviated the OA symptoms in experimental rat models.
BACKGROUND:Osteoarthritis (OA) is one of the complex diseases that affect a large population of the world. The aim of the current study was to explore the roles of taurine in OA rat models, and discover the related mechanisms. METHODS: OA rat models were established by anterior cruciate ligament transection (ACLT) plus medial meniscus resection (MMx) surgery on the right knees. Secondary mechanical allodynia, weight-bearing alterations and knee joint width were evaluated before surgery and every two weeks after surgery. At 14weeks, histopathological analysis was conducted on the knee joint cartilage. Protein amount of MMP-3 and CHOP was evaluated by western blot. RESULTS:Taurine injection after surgery significantly relieved the symptoms of OA in rat models in a dose-dependent and time-dependent manner, as shown by alleviation of secondary mechanical allodynia, decrease in hind limb weight-bearing alterations, and inhibited knee swelling. Moreover, histopathological analysis showed that taurine inhibited matrix loss and cartilage degeneration in a dose-dependent manner. Taurine administration strikingly suppressed the expression of matrix metalloproteinase-3 (MMP-3) and CHOP. CONCLUSION: These results indicated that taurine administration exhibited protective effects by inhibiting MMP-3 and CHOP expression, and subsequently alleviated the OA symptoms in experimental rat models.
Authors: Elizabeth Pérez-Hernández; Jesús Javier Pastrana-Carballo; Fernando Gómez-Chávez; Ramesh C Gupta; Nury Pérez-Hernández Journal: Endocrinol Metab (Seoul) Date: 2022-08-08