Qiu-Xia Yan1, Yi Ma, Run-Qiang Chen, Xiu-Qin Zhou, Jing Qiao, Ying-Jie Xian, Ling Feng, Cai-Rong Chen. 1. 1Center for Reproductive Medicine, Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People's Hospital, Qingyuan 511518,China; 2Department of Cellular Biology, Institute of Biological Medicine, Jinan University, Guangzhou 510632, China. E-mail: yanqiuxia1982@163.com.
Abstract
OBJECTIVE: To compare the expression of DKKL1 in ejaculated spermatozoa of normal fertile men and men with asthenospermia and investigate the role of DKKL1 in the pathogenesis of asthenospermia. METHODS: The characteristics of semen samples collected from normal fertile men and men with asthenospermia were analyzed using computer-assisted sperm analysis according to WHO criteria. The ejaculated sperms were isolated by Percoll discontinuous density gradients to detect the expression of DKKL1 mRNA and protein using real-time PCR and Western blotting. RESULTS: The expression of DKKL1 mRNA was significantly down-regulated by 11.1 times in asthenospermic men as compared with that in normal fertile men (P<0.01). Western blotting showed that the expression of DKKL1 protein was down-regulated by 2.4 times in asthenospermic men compared to normal fertile men. CONCLUSION: The expression of DKKL1, which may play an important role in sperm motility,is significantly decreased in ejaculated spermatozoa of men with asthenospermia.
OBJECTIVE: To compare the expression of DKKL1 in ejaculated spermatozoa of normal fertile men and men with asthenospermia and investigate the role of DKKL1 in the pathogenesis of asthenospermia. METHODS: The characteristics of semen samples collected from normal fertile men and men with asthenospermia were analyzed using computer-assisted sperm analysis according to WHO criteria. The ejaculated sperms were isolated by Percoll discontinuous density gradients to detect the expression of DKKL1 mRNA and protein using real-time PCR and Western blotting. RESULTS: The expression of DKKL1 mRNA was significantly down-regulated by 11.1 times in asthenospermic men as compared with that in normal fertile men (P<0.01). Western blotting showed that the expression of DKKL1 protein was down-regulated by 2.4 times in asthenospermic men compared to normal fertile men. CONCLUSION: The expression of DKKL1, which may play an important role in sperm motility,is significantly decreased in ejaculated spermatozoa of men with asthenospermia.
Authors: Liesbeth Visser; G Henrike Westerveld; Fang Xie; Saskia K M van Daalen; Fulco van der Veen; M Paola Lombardi; Sjoerd Repping Journal: Fertil Steril Date: 2011-01-21 Impact factor: 7.329
Authors: V Mitchell; J Sigala; F Jumeau; C Ballot; M C Peers; C Decanter; N Rives; A Perdrix; J-M Rigot; D Escalier Journal: Gynecol Obstet Fertil Date: 2012-11-20