Getinet Mekuriaw Tarekegn1,2, Xiao-Yang Ji3, Xue Bai3, Bin Liu4, Wenguang Zhang3, Josephine Birungi5, Appolinaire Djikeng6, Kassahun Tesfaye7. 1. Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala P.O. Box 7070, Sweden. 2. Department of Animal Production and Technology, Bahir Dar University, Bahir Dar P.O. Box 79, Ethiopia. 3. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China. 4. Nei Mongol BioNew Technology Co. Ltd, Hohhot 010020, China. 5. Biosciences Eastern and Central Africa (BecA) Hub - International Livestock Research Institute, PO Box 30709, Nairobi 00100, Kenya. 6. Centre for Tropical Livestock Genetics and Health, The University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, Scotland, UK. 7. Department of Microbial Cellular and Molecular Biology, Addis Ababa University, Addis Ababa P.O. Box, 1176, Ethiopia.
Abstract
OBJECTIVE: This study was carried out to assess the haplotype diversity and population dynamics in cattle populations of Ethiopia. METHODS: We sequenced the complete mitochondrial cytochrome b gene of 76 animals from five indigenous and one Holstein Friesian×Barka cross bred cattle populations. RESULTS: In the sequence analysis, 18 haplotypes were generated from 18 segregating sites and the average haplotype and nucleotide diversities were 0.7540±0.043 and 0.0010±0.000, respectively. The population differentiation analysis shows a weak population structure (4.55%) among the populations studied. Majority of the variation (95.45%) is observed by within populations. The overall average pair-wise distance (FST) was 0.049539 with the highest (FST = 0.1245) and the lowest (FST = 0.011) FST distances observed between Boran and Abigar, and Sheko and Abigar from the indigenous cattle, respectively. The phylogenetic network analysis revealed that all the haplotypes detected clustered together with the Bos taurus cattle and converged to a haplogroup. No haplotype in Ethiopian cattle was observed clustered with the reference Bos indicus group. The mismatch distribution analysis indicates a single population expansion event among the cattle populations. CONCLUSION: Overall, high haplotype variability was observed among Ethiopian cattle populations and they share a common ancestor with Bos taurus.
OBJECTIVE: This study was carried out to assess the haplotype diversity and population dynamics in cattle populations of Ethiopia. METHODS: We sequenced the complete mitochondrial cytochrome b gene of 76 animals from five indigenous and one Holstein Friesian×Barka cross bred cattle populations. RESULTS: In the sequence analysis, 18 haplotypes were generated from 18 segregating sites and the average haplotype and nucleotide diversities were 0.7540±0.043 and 0.0010±0.000, respectively. The population differentiation analysis shows a weak population structure (4.55%) among the populations studied. Majority of the variation (95.45%) is observed by within populations. The overall average pair-wise distance (FST) was 0.049539 with the highest (FST = 0.1245) and the lowest (FST = 0.011) FST distances observed between Boran and Abigar, and Sheko and Abigar from the indigenous cattle, respectively. The phylogenetic network analysis revealed that all the haplotypes detected clustered together with the Bos taurus cattle and converged to a haplogroup. No haplotype in Ethiopian cattle was observed clustered with the reference Bos indicus group. The mismatch distribution analysis indicates a single population expansion event among the cattle populations. CONCLUSION: Overall, high haplotype variability was observed among Ethiopian cattle populations and they share a common ancestor with Bos taurus.
Entities:
Keywords:
Cytochrome b; Ethiopian Cattle; Haplotype Diversity; Population Expansion
Authors: C S Troy; D E MacHugh; J F Bailey; D A Magee; R T Loftus; P Cunningham; A T Chamberlain; B C Sykes; D G Bradley Journal: Nature Date: 2001-04-26 Impact factor: 49.962
Authors: H Mannen; M Kohno; Y Nagata; S Tsuji; D G Bradley; J S Yeo; D Nyamsamba; Y Zagdsuren; M Yokohama; K Nomura; T Amano Journal: Mol Phylogenet Evol Date: 2004-08 Impact factor: 4.286