Tuning of elasticity and surface properties of hydrogel cell culture substrates by simple chemical approach.

When designing materials for tissue engineering applications various parameters characterizing both materials and tissue have to be taken into account. The characteristics such as chemistry, elasticity, wettability, roughness and morphology of the substrate's surface have significant impact on cell behavior. The paper presents biopolymer (collagen/chitosan) based hydrogel materials with tunable elasticity and surface properties useful for fabrication of substrates for cell culture. Using simple chemical approach involving the change in concentration of crosslinking agent (genipin) and composition of the reaction mixture the hydrogels characterized with various features were obtained. Detailed analysis of morphology, topography, roughness and elasticity of surface performed using Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM) and rheological measurements has shown that the topographical aspects and roughness parameter can be modulated in nanoscale regime (13-47 nm). Substrate's elasticity could be modified in a wide range (0.2-270 kPa). Biological in vitro studies on fibroblasts behavior revealed that the materials prepared provide satisfactory conditions for cell culture, ensuring their high viability, good adhesion and normal morphology. The genipin crosslinked collagen-chitosan hydrogels characterized by denser fiber structure, higher elasticity and lower surface roughness are the most attractive supports for fibroblasts cultivation. The results obtained indicate that the properties of the materials developed can be easily tailored to the needs of the given type of cells.