Literature DB >> 29633966

Crystal structure of pyrimidine-nucleoside phosphorylase from Bacillus subtilis in complex with imidazole and sulfate.

V V Balaev1, I I Prokofev1, A G Gabdoulkhakov1, C Betzel2, A A Lashkov1.   

Abstract

Pyrimidine-nucleoside phosphorylase catalyzes the phosphorolytic cleavage of thymidine and uridine with equal activity. Investigation of this protein is essential for anticancer drug design. Here, the structure of this protein from Bacillus subtilis in complex with imidazole and sulfate is reported at 1.9 Å resolution, which is an improvement on the previously reported structure at 2.6 Å resolution. The localization and position of imidazole in the nucleoside-binding site reflects the possible binding of ligands that possess an imidazole ring.

Entities:  

Keywords:  Bacillus subtilis; X-ray analysis; imidazole; nucleoside phosphorylases; nucleosides; protein crystallography; pyrimidine-nucleoside phosphorylase

Mesh：

Substances：

Year:  2018        PMID： 29633966      PMCID： PMC5894104          DOI： 10.1107/S2053230X18002935

Source DB:  PubMed          Journal:  Acta Crystallogr F Struct Biol Commun        ISSN： 2053-230X            Impact factor:   1.056


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