Literature DB >> 29633214

Fabrication and Operation of Microfluidic Hanging-Drop Networks.

Patrick M Misun1, Axel K Birchler2, Moritz Lang3, Andreas Hierlemann2, Olivier Frey4.   

Abstract

The hanging-drop network (HDN) is a technology platform based on a completely open microfluidic network at the bottom of an inverted, surface-patterned substrate. The platform is predominantly used for the formation, culturing, and interaction of self-assembled spherical microtissues (spheroids) under precisely controlled flow conditions. Here, we describe design, fabrication, and operation of microfluidic hanging-drop networks.

Entities:  

Keywords:  3D tissue; Body-on-a-chip; Long-term culturing; Microfluidics; Microtissue; Organ-on-a-chip; Perfusion; Scaffold-free; Spheroid; Tissue engineering

Mesh：

Year:  2018        PMID： 29633214      PMCID： PMC7612358          DOI： 10.1007/978-1-4939-7792-5_15

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  7 in total

1.  Use of YouScope to implement systematic microscopy protocols.

Authors:  Moritz Lang; Fabian Rudolf; Jörg Stelling
Journal:  Curr Protoc Mol Biol       Date:  2012-04

2.  Reconfigurable microfluidic hanging drop network for multi-tissue interaction and analysis.

Authors:  Olivier Frey; Patrick M Misun; David A Fluri; Jan G Hengstler; Andreas Hierlemann
Journal:  Nat Commun       Date:  2014-06-30       Impact factor: 14.919

3.  Seamless Combination of Fluorescence-Activated Cell Sorting and Hanging-Drop Networks for Individual Handling and Culturing of Stem Cells and Microtissue Spheroids.

Authors:  Axel Birchler; Mischa Berger; Verena Jäggin; Telma Lopes; Martin Etzrodt; Patrick Mark Misun; Maria Pena-Francesch; Timm Schroeder; Andreas Hierlemann; Olivier Frey
Journal:  Anal Chem       Date:  2016-01-06       Impact factor: 6.986

4.  Method for generation of homogeneous multicellular tumor spheroids applicable to a wide variety of cell types.

Authors:  Jens M Kelm; Nicholas E Timmins; Catherine J Brown; Martin Fussenegger; Lars K Nielsen
Journal:  Biotechnol Bioeng       Date:  2003-07-20       Impact factor: 4.530

5.  Adding the 'heart' to hanging drop networks for microphysiological multi-tissue experiments.

Authors:  Saeed Rismani Yazdi; Amir Shadmani; Sebastian C Bürgel; Patrick M Misun; Andreas Hierlemann; Olivier Frey
Journal:  Lab Chip       Date:  2015-09-24       Impact factor: 6.799

6.  Electrical Impedance Spectroscopy for Microtissue Spheroid Analysis in Hanging-Drop Networks.

Authors:  Yannick R F Schmid; Sebastian C Bürgel; Patrick M Misun; Andreas Hierlemann; Olivier Frey
Journal:  ACS Sens       Date:  2016-07-18       Impact factor: 7.711

7.  Multi-analyte biosensor interface for real-time monitoring of 3D microtissue spheroids in hanging-drop networks.

Authors:  Patrick M Misun; Jörg Rothe; Yannick R F Schmid; Andreas Hierlemann; Olivier Frey
Journal:  Microsyst Nanoeng       Date:  2016-06-06       Impact factor: 7.127
  7 in total
  3 in total

1.  In Vitro Platform for Studying Human Insulin Release Dynamics of Single Pancreatic Islet Microtissues at High Resolution.

Authors:  Patrick M Misun; Burçak Yesildag; Felix Forschler; Aparna Neelakandhan; Nassim Rousset; Adelinn Biernath; Andreas Hierlemann; Olivier Frey
Journal:  Adv Biosyst       Date:  2020-01-29

2.  A Microfluidic Hanging-Drop-Based Islet Perifusion System for Studying Glucose-Stimulated Insulin Secretion From Multiple Individual Pancreatic Islets.

Authors:  Patricia Wu Jin; Nassim Rousset; Andreas Hierlemann; Patrick M Misun
Journal:  Front Bioeng Biotechnol       Date:  2021-05-12

3.  Modeling and measuring glucose diffusion and consumption by colorectal cancer spheroids in hanging drops using integrated biosensors.

Authors:  Nassim Rousset; Rubén López Sandoval; Mario Matteo Modena; Andreas Hierlemann; Patrick M Misun
Journal:  Microsyst Nanoeng       Date:  2022-02-01       Impact factor: 7.127
  3 in total

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