| Literature DB >> 29633204 |
Xumei Gao, Celine Stoecklin, Yue Zhang1, Zhe Weng1, Richard De Mets1, Gianluca Grenci1, Virgile Viasnoff2,3,4.
Abstract
We present here a method to create arrays of microcavities that can be differentially coated on their bottom, side, and top with different proteins. These cavities range in size from single cell to multicellular aggregate. We provide detailed protocols to create such arrays with some variations using different materials and different coating proteins. The use of such cavities as bona fide artificial microniches to mimic cellular microenvironments has been already established and is referenced.Keywords: Array; Artificial microniche; Cellular microenvironment; Microcavities
Mesh：
Year: 2018 PMID： 29633204 DOI： 10.1007/978-1-4939-7792-5_5
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745