Z-G Gu1, G-H Shen, J-H Lang, W-X Huang, Z-H Qian, J Qiu. 1. Department of Gastrointestinal Surgery, The First People's Hospital of Wujiang District Suzhou, Suzhou, China. qiujie13316@163.com.
Abstract
OBJECTIVE: To investigate the effect of long non-coding RNA URHC on the proliferation, apoptosis and invasion of colorectal cancer cells. PATIENTS AND METHODS: The expression of lncRNA-URHC in tissues and cells was tested by Real-time quantitative PCR. The expression of lncRNA-URHC was down-regulated by RNA interference (siRNA). The Real-time quantitative polymerase chain reaction (PCR) method was used to detect the interference efficiency. Cell counting kit-8 (CCK-8), flow cytometry, and transwell were used to detect the effect of lncRNA-URHC on the proliferation, apoptosis and invasion of colorectal cancer cells. The effect of lncRNA-URHC on epithelial-mesenchymal transition (EMT)-related markers was detected by Western blot. RESULTS: LncRNA-URHC expression was significantly increased in colorectal cancer cells compared with normal cells, and the expression of lncRNA-URHC in colorectal cancer cells was higher than that in the normal cell. After down-regulated the expression of lncRNA-URHC, the proliferation and invasion of colorectal cancer cells were decreased, while cells apoptosis was promoted. Down-regulation of lncRNA-URHC could enhance the expression of E-cadherin and reduce the expression of N-cadherin, vimentin and snail. CONCLUSIONS: Down-regulation of lncRNA-URHC can inhibit the progression of colorectal cancer.
OBJECTIVE: To investigate the effect of long non-coding RNA URHC on the proliferation, apoptosis and invasion of colorectal cancer cells. PATIENTS AND METHODS: The expression of lncRNA-URHC in tissues and cells was tested by Real-time quantitative PCR. The expression of lncRNA-URHC was down-regulated by RNA interference (siRNA). The Real-time quantitative polymerase chain reaction (PCR) method was used to detect the interference efficiency. Cell counting kit-8 (CCK-8), flow cytometry, and transwell were used to detect the effect of lncRNA-URHC on the proliferation, apoptosis and invasion of colorectal cancer cells. The effect of lncRNA-URHC on epithelial-mesenchymal transition (EMT)-related markers was detected by Western blot. RESULTS: LncRNA-URHC expression was significantly increased in colorectal cancer cells compared with normal cells, and the expression of lncRNA-URHC in colorectal cancer cells was higher than that in the normal cell. After down-regulated the expression of lncRNA-URHC, the proliferation and invasion of colorectal cancer cells were decreased, while cells apoptosis was promoted. Down-regulation of lncRNA-URHC could enhance the expression of E-cadherin and reduce the expression of N-cadherin, vimentin and snail. CONCLUSIONS: Down-regulation of lncRNA-URHC can inhibit the progression of colorectal cancer.