Anna L Luss1, Pavel P Kulikov1, Sven B Romme2, Camilla L Andersen2, Cristian P Pennisi3, Anca O Docea4, Andrey N Kuskov1, Kelly Velonia5, Yaroslav O Mezhuev1, Mikhail I Shtilman1,6, Aristidis M Tsatsakis7,6, Leonid Gurevich2. 1. Department of Biomaterials, Dmitry Mendeleev University of Chemical Technology of Russia, Miusskaya sq 9, 125047 Moscow, Russia. 2. Department of Materials & Production, Aalborg University, Skjernvej 4A, 9220, Aalborg, Denmark. 3. Department of Health Science & Technology, Aalborg University, Fredrik Bajers Vej 3, 9220, Aalborg, Denmark. 4. Department of Toxicology, Faculty of Pharmacy, University of Medicine & Pharmacy, 2 Petru Rares, 200349, Craiova, Romania. 5. Department of Materials Science & Technology, School of Sciences & Engineering, University of Crete, University Campus Voutes, 71003 Heraklion, Crete, Greece. 6. Bauman Moscow State Technical University, Center Composites of Russia, 2nd Baumanskaya 5, 105005 Moscow, Russia. 7. Laboratory of Toxicology, Medical School, University of Crete, Voutes, Heraklion Crete 71003, Greece.
Abstract
AIM: Ability to deliver drugs into the cell nuclei can significantly increase the efficacy of cancer therapies, in particular in the case of multidrug-resistant cancer Results: Polymer nanocarriers based on amphiphilic thiooctadecyl-terminated poly-N-vinyl-2-pyrrolidone were produced and loaded with a model hydrophobic drug, curcumin. Two commonly used loading approaches - emulsification and ultrasonic dispersion - were found to lead to two different size distributions with distinctively different biological effect. While nanocarriers produced via the emulsion method penetrated cells by dynamin-dependent endocytic mechanisms, sub-100 nm dispersion-produced nanocarriers were capable of crossing the membranes via biologically independent mechanisms. CONCLUSION: This finding opens an intriguing possibility of intranuclear delivery by merely tailoring the size of polymeric carriers, thus promising a new approach for cancer therapies.
AIM: Ability to deliver drugs into the cell nuclei can significantly increase the efficacy of cancer therapies, in particular in the case of multidrug-resistant cancer Results: Polymer nanocarriers based on amphiphilic thiooctadecyl-terminated poly-N-vinyl-2-pyrrolidone were produced and loaded with a model hydrophobic drug, curcumin. Two commonly used loading approaches - emulsification and ultrasonic dispersion - were found to lead to two different size distributions with distinctively different biological effect. While nanocarriers produced via the emulsion method penetrated cells by dynamin-dependent endocytic mechanisms, sub-100 nm dispersion-produced nanocarriers were capable of crossing the membranes via biologically independent mechanisms. CONCLUSION: This finding opens an intriguing possibility of intranuclear delivery by merely tailoring the size of polymeric carriers, thus promising a new approach for cancer therapies.
Entities:
Keywords:
PVP; atomic force microscopy; curcumin; fluorescence microscopy; intranuclear drug delivery; nanocarrier
Authors: Natalia G Menzyanova; Svetlana А Pyatina; Elena D Nikolaeva; Alexander V Shabanov; Ivan V Nemtsev; Dmitry P Stolyarov; Dmitry B Dryganov; Eugene V Sakhnov; Ekaterina I Shishatskaya Journal: Toxicol Rep Date: 2018-11-16
Authors: Muhammad Athar Abbasi; Seon-Mi Yu; Sabahat Zahra Siddiqui; Song Ja Kim; Hussain Raza; Mubashir Hassan; Abdul Rehman Sadiq Butt; Syed Anan Ali Shah; Sung-Yum Seo Journal: Toxicol Rep Date: 2019-08-26
Authors: Anca Oana Docea; Daniela Calina; Ana Maria Buga; Ovidiu Zlatian; M M B Paoliello; George Dan Mogosanu; Costin Teodor Streba; Elena Leocadia Popescu; Alexandra Elena Stoica; Alexandra Catalina Bîrcă; Bogdan Ștefan Vasile; Alexandru Mihai Grumezescu; Laurentiu Mogoanta Journal: Int J Mol Sci Date: 2020-02-12 Impact factor: 5.923