Vanja P Ničković1, Tatjana Novaković2, Slavica Lazarević2, Ljiljana Šulović2, Zorica Živković2, Jovan Živković2, Bojan Mladenović3, Nikola M Stojanović4, Vladmir Petrović5, Dušan T Sokolović6. 1. Clinical-Hospital Center, Pristina, Gracanica, Serbia. 2. Medical faculty, University of Pristina, Kosovska Mitrovica, Serbia. 3. Clinic for Gastroenterology, Clinical Center Niš, Serbia. 4. Faculty of Medicine, University of Niš, Zorana Đinđića 81, 18000 Niš, Serbia. 5. Institute of Histology, Faculty of Medicine, University of Niš, Zorana Đinđića 81, 18000 Niš, Serbia. 6. Department of Biochemistry, Faculty of Medicine, University of Niš, Zorana Đinđića 81, 18000 Niš, Serbia. Electronic address: dusantsokolovic@gmail.com.
Abstract
AIMS: The present study was designed to compare the ameliorating potential of pre- and post-treatments with melatonin, a potent natural antioxidant, in the carbon tetrachloride-induced rat liver damage model by tracking changes in enzymatic and non-enzymatic liver tissue defense parameters, as well as in the occurring pathohistological changes. MAIN METHODS: Rats from two experimental groups were treated with melatonin before and after CCl4 administration, while the controls, negative and positive, received vehicle/melatonin and CCl4, respectively. Serum levels of transaminases, alkaline phosphates, γ-GT, bilirubin, and albumin, as well as a wide panel of oxidative stress-related parameters in liver tissue, were determined in all experimental animals. Liver tissue specimens were stained with hematoxylin and eosin and further evaluated for morphological changes. KEY FINDINGS: Both pre- and post-treatment with melatonin prevented a CCl4-induced increase in serum (ALT, AST, and γ-GT) and tissue (MDA and XO) liver damage markers and a decrease in the tissue total antioxidant capacity, in both enzymatic and non-enzymatic systems. The intensity of pathological changes, hepatocyte vacuolar degeneration, necrosis and inflammatory cell infiltration, was suppressed by the treatment with melatonin. SIGNIFICANCE: In conclusion, melatonin, especially as a post-intoxication treatment, attenuated CCl4-induced liver oxidative damage, increased liver antioxidant capacities and improved liver microscopic appearance. The results are of interest due to the great protective potential of melatonin that was even demonstrated to be stronger if applied after the tissue damage.
AIMS: The present study was designed to compare the ameliorating potential of pre- and post-treatments with melatonin, a potent natural antioxidant, in the carbon tetrachloride-induced ratliver damage model by tracking changes in enzymatic and non-enzymatic liver tissue defense parameters, as well as in the occurring pathohistological changes. MAIN METHODS:Rats from two experimental groups were treated with melatonin before and after CCl4 administration, while the controls, negative and positive, received vehicle/melatonin and CCl4, respectively. Serum levels of transaminases, alkaline phosphates, γ-GT, bilirubin, and albumin, as well as a wide panel of oxidative stress-related parameters in liver tissue, were determined in all experimental animals. Liver tissue specimens were stained with hematoxylin and eosin and further evaluated for morphological changes. KEY FINDINGS: Both pre- and post-treatment with melatonin prevented a CCl4-induced increase in serum (ALT, AST, and γ-GT) and tissue (MDA and XO) liver damage markers and a decrease in the tissue total antioxidant capacity, in both enzymatic and non-enzymatic systems. The intensity of pathological changes, hepatocyte vacuolar degeneration, necrosis and inflammatory cell infiltration, was suppressed by the treatment with melatonin. SIGNIFICANCE: In conclusion, melatonin, especially as a post-intoxication treatment, attenuated CCl4-induced liver oxidative damage, increased liver antioxidant capacities and improved liver microscopic appearance. The results are of interest due to the great protective potential of melatonin that was even demonstrated to be stronger if applied after the tissue damage.
Authors: Dušan T Sokolović; Ljubiša Lilić; Vesko Milenković; Rade Stefanović; Tatjana Popović Ilić; Branimir Mekić; Igor Ilić; Nikola M Stojanović; Ivan R Ilić Journal: Saudi Pharm J Date: 2018-05-31 Impact factor: 4.330