Jae-Hyun Jung1, Dong-Hyun Kim2, Kyung-Hyeon Yoo3, Seog-Young Yoon3, Yeon Kim4, Moon-Kyoung Bae4, Jin Chung5, Ching-Chang Ko6, Yong Hoon Kwon7, Yong-Il Kim8,9. 1. Department of Orthodontics, Dental Research Institute, Pusan National University Dental Hospital, Geumoro 20, Mulgeum, Yangsan, 50612, Republic of Korea. 2. R&D Center, Upex. Med Co., Ltd., Hagui-ro 282, Dongan-gu, Anyang, Gyeonggi-do, 14056, Republic of Korea. 3. School of Materials Science and Engineering, Pusan National University, Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan, 46241, Republic of Korea. 4. Department of Oral Physiology, School of Dentistry, Pusan National University, Busandaehak-ro 49, Mulgeum, Yangsan, 50612, Republic of Korea. 5. Department of Oral Microbiology, School of Dentistry, Pusan National University, Busandaehak-ro 49, Mulgeum, Yangsan, 50612, Republic of Korea. 6. Department of Orthodontics, School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27516, USA. 7. Department of Dental Materials, Pusan National University, Busandaehak-ro 49, Mulgeum, Yangsan, 50612, Republic of Korea. y0k0916@pusan.ac.kr. 8. Department of Orthodontics, Dental Research Institute, Pusan National University Dental Hospital, Geumoro 20, Mulgeum, Yangsan, 50612, Republic of Korea. kimyongil@pusan.ac.kr. 9. Institute of Translational Dental Sciences, School of Dentistry, Pusan National University, Busandaehak-ro 49, Mulgeum, Yangsan, 50612, Republic of Korea. kimyongil@pusan.ac.kr.
Abstract
OBJECTIVES: To synthesize a silver-doped bioactive glass/mesoporous silica nanoparticle (Ag-BGN@MSN), as well as to investigate its effects on dentinal tubule occlusion, microtensile bond strength (MTBS), and antibacterial activity. MATERIALS AND METHODS: Ag-BGN@MSN was synthesized using a modified "quick alkali-mediated sol-gel" method. Demineralized tooth disc models were made and divided into four groups; the following treatments were then applied: group 1-no treatment, group 2-bioglass, group 3-MSN, group 4-Ag-BGN@MSN. Next, four discs were selected from each group and soaked into 6 wt% citric acid to test acid-resistant stability. Dentinal tubule occlusion, as well as the occlusion ratio, was observed using field-emission scanning electron microscopy. The MTBS was also measured to evaluate the desensitizing effect of the treatments. Cytotoxicity was examined using the MTT assay. Antibacterial activity was detected against Lactobacillus casei, and ion dissolution was evaluated using inductively coupled plasma optical emission spectrometry. RESULTS: Ag-BGN@MSN effectively occluded the dentinal tubule and formed a membrane-like layer. After the acid challenge, Ag-BGN@MSN had the highest rate of dentinal tubule occlusion. There were no significant differences in MTBS among the four groups (P > 0.05). All concentrations of Ag-BGN@MSN used had a relative cell viability above 72%. CONCLUSIONS: Ag-BGN@MSN was successfully fabricated using a modified sol-gel method. The Ag-BGN@MSN biocomposite effectively occluded dentinal with acid-resistant stability, did not decrease bond strength in self-etch adhesive system, had low cytotoxicity, and antibacterial effect. CLININAL RELEVANCE: Dentinal tubule sealing induced by Ag-BGN@MSN biocomposite with antibacterial effect is likely to increase long-term stability in DH.
OBJECTIVES: To synthesize a silver-doped bioactive glass/mesoporous silica nanoparticle (Ag-BGN@MSN), as well as to investigate its effects on dentinal tubule occlusion, microtensile bond strength (MTBS), and antibacterial activity. MATERIALS AND METHODS: Ag-BGN@MSN was synthesized using a modified "quick alkali-mediated sol-gel" method. Demineralized tooth disc models were made and divided into four groups; the following treatments were then applied: group 1-no treatment, group 2-bioglass, group 3-MSN, group 4-Ag-BGN@MSN. Next, four discs were selected from each group and soaked into 6 wt% citric acid to test acid-resistant stability. Dentinal tubule occlusion, as well as the occlusion ratio, was observed using field-emission scanning electron microscopy. The MTBS was also measured to evaluate the desensitizing effect of the treatments. Cytotoxicity was examined using the MTT assay. Antibacterial activity was detected against Lactobacillus casei, and ion dissolution was evaluated using inductively coupled plasma optical emission spectrometry. RESULTS: Ag-BGN@MSN effectively occluded the dentinal tubule and formed a membrane-like layer. After the acid challenge, Ag-BGN@MSN had the highest rate of dentinal tubule occlusion. There were no significant differences in MTBS among the four groups (P > 0.05). All concentrations of Ag-BGN@MSN used had a relative cell viability above 72%. CONCLUSIONS: Ag-BGN@MSN was successfully fabricated using a modified sol-gel method. The Ag-BGN@MSN biocomposite effectively occluded dentinal with acid-resistant stability, did not decrease bond strength in self-etch adhesive system, had low cytotoxicity, and antibacterial effect. CLININAL RELEVANCE: Dentinal tubule sealing induced by Ag-BGN@MSN biocomposite with antibacterial effect is likely to increase long-term stability in DH.