Literature DB >> 2961614

ATPase activity of the microvillar 110 kDa polypeptide-calmodulin complex is activated in Mg2+ and inhibited in K+-EDTA by F-actin.

J Krizek1, L M Coluccio, A Bretscher.   

Abstract

Highly purified microvillar 110 kDa polypeptide-calmodulin (110K-cam) complex was confirmed to have ATPase activities characteristic of a myosin. The effect of F-actin on these activities was investigated. The Mg2+-ATPase is activated about 2-fold by F-actin in a dose-dependent fashion, whereas the K+-EDTA-ATPase is inhibited by greater than 90% by F-actin. These data provide evidence for a functional relationship between the ATPase activity of 110K-cam and its interaction with F-actin. They also extend the similarities between 110K-cam and myosin. The results suggest that higher cells contain in addition to myosin a second class of myosin-like molecules represented by 110K-cam.

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Year:  1987        PMID： 2961614     DOI： 10.1016/0014-5793(87)81172-9

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  9 in total

1.  Unipolar localization and ATPase activity of IcsA, a Shigella flexneri protein involved in intracellular movement.

Authors:  M B Goldberg; O Bârzu; C Parsot; P J Sansonetti
Journal:  J Bacteriol       Date:  1993-04       Impact factor: 3.490

2.  Binding of brush border myosin I to phospholipid vesicles.

Authors:  S M Hayden; J S Wolenski; M S Mooseker
Journal:  J Cell Biol       Date:  1990-08       Impact factor: 10.539

3.  Calmodulin dissociation regulates brush border myosin I (110-kD-calmodulin) mechanochemical activity in vitro.

Authors:  K Collins; J R Sellers; P Matsudaira
Journal:  J Cell Biol       Date:  1990-04       Impact factor: 10.539

4.  Reassociation of microvillar core proteins: making a microvillar core in vitro.

Authors:  L M Coluccio; A Bretscher
Journal:  J Cell Biol       Date:  1989-02       Impact factor: 10.539

5.  The 110-kD protein-calmodulin complex of the intestinal microvillus (brush border myosin I) is a mechanoenzyme.

Authors:  M S Mooseker; T R Coleman
Journal:  J Cell Biol       Date:  1989-06       Impact factor: 10.539

6.  Characterization of intestinal microvillar membrane disks: detergent-resistant membrane sheets enriched in associated brush border myosin I (110K-calmodulin).

Authors:  M S Mooseker; K A Conzelman; T R Coleman; J E Heuser; M P Sheetz
Journal:  J Cell Biol       Date:  1989-09       Impact factor: 10.539

7.  Structural and immunological characterization of the myosin-like 110-kD subunit of the intestinal microvillar 110K-calmodulin complex: evidence for discrete myosin head and calmodulin-binding domains.

Authors:  J M Carboni; K A Conzelman; R A Adams; D A Kaiser; T D Pollard; M S Mooseker
Journal:  J Cell Biol       Date:  1988-11       Impact factor: 10.539

8.  Rapid phosphorylation and reorganization of ezrin and spectrin accompany morphological changes induced in A-431 cells by epidermal growth factor.

Authors:  A Bretscher
Journal:  J Cell Biol       Date:  1989-03       Impact factor: 10.539

9.  Partial deduced sequence of the 110-kD-calmodulin complex of the avian intestinal microvillus shows that this mechanoenzyme is a member of the myosin I family.

Authors:  A Garcia; E Coudrier; J Carboni; J Anderson; J Vandekerkhove; M Mooseker; D Louvard; M Arpin
Journal:  J Cell Biol       Date:  1989-12       Impact factor: 10.539
  9 in total

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