Molecular epidemiological survey of canine parvovirus in domestic dogs in four provinces, China.

Canine parvovirus (CPV) can cause severe disease in animals, especially in dogs, and continuously generates new variants. In this study, the complete VP2 genes of 59 CPV isolates from clinical diseased dogs between 2015 and 2016 in 4 Chinese provinces were sequenced and analyzed. The results showed that new CPV-2a was still the prominent CPV genotype, followed by CPV-2c and new CPV-2b. CPV-2c with Ala5Gly and Gln370Arg mutations was first detected in Henan, Guangxi and Jiangsu provinces in China. A phylogenetic tree based on VP2 sequences showed that all isolates in this study formed a close lineage which separated from foreign isolates. The above results indicated that point mutations in VP2 were constantly occurring along with the widespread of CPV in China.