Literature DB >> 29605911

Characterizing Protein-Protein Interactions Using Deep Sequencing Coupled to Yeast Surface Display.

Angelica V Medina-Cucurella1, Timothy A Whitehead2,3.   

Abstract

In this chapter, we discuss a method to determine the affinity and specificity of nearly all single-point mutants for a full-length protein binder. This method combines deep sequencing, comprehensive mutagenesis, yeast surface display, and fluorescence-activated cell sorting. This approach has been used to study sequence-function relationships for protein-protein interactions. The data can be used to determine the fine conformational epitope on the protein binder.

Entities:  

Keywords:  Conformational epitope mapping; Deep sequencing; FACS; Nicking mutagenesis; Yeast surface display

Mesh：

Substances：

Year:  2018        PMID： 29605911      PMCID： PMC6664802          DOI： 10.1007/978-1-4939-7759-8_7

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  12 in total

1.  Mutational fitness landscapes reveal genetic and structural improvement pathways for a vaccine-elicited HIV-1 broadly neutralizing antibody.

Authors:  Bharat Madan; Baoshan Zhang; Kai Xu; Cara W Chao; Sijy O'Dell; Jacy R Wolfe; Gwo-Yu Chuang; Ahmed S Fahad; Hui Geng; Rui Kong; Mark K Louder; Thuy Duong Nguyen; Reda Rawi; Arne Schön; Zizhang Sheng; Rajani Nimrania; Yiran Wang; Tongqing Zhou; Bob C Lin; Nicole A Doria-Rose; Lawrence Shapiro; Peter D Kwong; Brandon J DeKosky
Journal:  Proc Natl Acad Sci U S A       Date:  2021-03-09       Impact factor: 11.205

2.  Feline Interleukin-31 Shares Overlapping Epitopes with the Oncostatin M Receptor and IL-31RA.

Authors:  Angelica V Medina-Cucurella; Gary F Bammert; William Dunkle; Christopher Javens; Yaqi Zhu; Veronica T Mutchler; Janet T Teel; Caitlin A Stein; Steve A Dunham; Timothy A Whitehead
Journal:  Biochemistry       Date:  2020-06-04       Impact factor: 3.162

3.  Incorporating, Quantifying, and Leveraging Noncanonical Amino Acids in Yeast.

Authors:  Jessica T Stieglitz; James A Van Deventer
Journal:  Methods Mol Biol       Date:  2022

4.  Yeast Surface Display: New Opportunities for a Time-Tested Protein Engineering System.

Authors:  Maryam Raeeszadeh-Sarmazdeh; Eric T Boder
Journal:  Methods Mol Biol       Date:  2022

5.  Stabilization of the SARS-CoV-2 receptor binding domain by protein core redesign and deep mutational scanning.

Authors:  Alison C Leonard; Jonathan J Weinstein; Paul J Steiner; Annette H Erbse; Sarel J Fleishman; Timothy A Whitehead
Journal:  Protein Eng Des Sel       Date:  2022-02-17       Impact factor: 1.952

6.  An antibody targeting type III secretion system induces broad protection against Salmonella and Shigella infections.

Authors:  Raphaël Sierocki; Bakhos Jneid; Maria Lucia Orsini Delgado; Marc Plaisance; Bernard Maillère; Hervé Nozach; Stéphanie Simon
Journal:  PLoS Negl Trop Dis       Date:  2021-03-12

7.  Stabilization of the SARS-CoV-2 Receptor Binding Domain by Protein Core Redesign and Deep Mutational Scanning.

Authors:  Alison C Leonard; Jonathan J Weinstein; Paul J Steiner; Annette H Erbse; Sarel J Fleishman; Timothy A Whitehead
Journal:  bioRxiv       Date:  2021-11-24

8.  Antibody screening at reduced pH enables preferential selection of potently neutralizing antibodies targeting SARS-CoV-2.

Authors:  Bharat Madan; Eswar R Reddem; Pengfei Wang; Ryan G Casner; Manoj S Nair; Yaoxing Huang; Ahmed S Fahad; Matheus Oliveira de Souza; Bailey B Banach; Sheila N López Acevedo; Xiaoli Pan; Rajani Nimrania; I-Ting Teng; Fabiana Bahna; Tongqing Zhou; Baoshan Zhang; Michael T Yin; David D Ho; Peter D Kwong; Lawrence Shapiro; Brandon J DeKosky
Journal:  AIChE J       Date:  2021-10-04       Impact factor: 3.993

9.  Characterization of Individual Human Antibodies That Bind Pertussis Toxin Stimulated by Acellular Immunization.

Authors:  Edith Acquaye-Seedah; Elizabeth E Reczek; Hugh H Russell; Andrea M DiVenere; Sara O Sandman; Joseph H Collins; Caitlin A Stein; Timothy A Whitehead; Jennifer A Maynard
Journal:  Infect Immun       Date:  2018-05-22       Impact factor: 3.441

10.  Identification of SARS-CoV-2 S RBD escape mutants using yeast screening and deep mutational scanning.

Authors:  Cyrus M Haas; Irene M Francino-Urdaniz; Paul J Steiner; Timothy A Whitehead
Journal:  STAR Protoc       Date:  2021-09-22
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