Nobuhiro Suzumori1, Akihiko Sekizawa2, Takeshi Ebara3, Osamu Samura4, Aiko Sasaki5, Rina Akaishi5, Seiji Wada5, Haruka Hamanoue6, Fumiki Hirahara6, Haruna Izumi6, Hideaki Sawai7, Hiroaki Nakamura8, Takahiro Yamada9, Kiyonori Miura10, Hideaki Masuzaki10, Takahiro Yamashita11, Takashi Okai11, Yoshimasa Kamei12, Akira Namba12, Jun Murotsuki13, Tomohiro Tanemoto4, Akimune Fukushima14, Kazufumi Haino15, Shinya Tairaku16, Keiichi Matsubara17, Kazutoshi Maeda18, Takashi Kaji19, Masanobu Ogawa20, Hisao Osada21, Haruki Nishizawa22, Yoko Okamoto23, Takeshi Kanagawa23, Aiko Kakigano24, Michihiro Kitagawa25, Masaki Ogawa26, Shunichiro Izumi27, Yukiko Katagiri28, Naoki Takeshita28, Yasuyo Kasai29, Katsuhiko Naruse30, Reiko Neki31, Hisashi Masuyama32, Maki Hyodo33, Yukie Kawano34, Takashi Ohba35, Kiyotake Ichizuka36, Takeshi Nagamatsu37, Atsushi Watanabe38, Nahoko Shirato2, Junko Yotsumoto39, Miyuki Nishiyama5, Tatsuko Hirose2, Haruhiko Sago2. 1. Department of Obstetrics and Gynecology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan. Electronic address: og.n.suz@med.nagoya-cu.ac.jp. 2. Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan. 3. Department of Occupational and Environmental Health, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan. 4. Department of Obstetrics and Gynecology, Jikei University School of Medicine, Tokyo, Japan. 5. Center of Maternal-Fetal, Neonatal and Reproductive Medicine, National Center for Child Health and Development, Tokyo, Japan. 6. Department of Clinical Genetics, Yokohama City University Hospital, Yokohama, Japan. 7. Department of Obstetrics and Gynecology, Hyogo College of Medicine, Nishinomiya, Japan. 8. Department of Obstetrics, Osaka City General Hospital, Osaka, Japan. 9. Department of Obstetrics and Gynecology, Hokkaido University Graduate School of Medicine, Sapporo, Japan. 10. Department of Obstetrics and Gynecology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan. 11. Maternal and Child Health Center, Aiiku Hospital, Tokyo, Japan. 12. Departments of Obstetrics and Gynecology, Saitama Medical University School of Medicine, Saitama, Japan. 13. Department of Maternal and Fetal Medicine, Tohoku University Graduate School of Medicine, Miyagi Children's Hospital, Sendai, Japan. 14. Department of Clinical Genetics, School of Medicine, Iwate Medical University, Morioka, Morioka, Japan. 15. Department of Obstetrics and Gynecology, Niigata University Medical and Dental Hospital, Niigata, Japan. 16. Department of Obstetrics and Gynecology, Kobe University Graduate School of Medicine, Kobe, Japan. 17. Department of Obstetrics and Gynecology, Ehime University School of Medicine, Matsuyama, Japan. 18. Department of Obstetrics and Gynecology, Shikoku Medical Center for Children and Adults, Kagawa, Japan. 19. Department of Obstetrics and Gynecology, The University of Tokushima, Faculty of Medicine, Tokushima, Japan. 20. Department of Obstetrics and Gynecology, Clinical Research Institute, National Hospital Organization Kyushu Medical Center, Fukuoka, Japan. 21. Department of Maternal-fetal Medicine, Chiba University Hospital, Chiba, Japan. 22. Department of Obstetrics and Gynecology, Fujita Health University, Aichi, Japan. 23. Department of Obstetrics, Osaka Medical Center and Research Institute for Maternal and Child Health, Osaka, Japan. 24. Department of Obstetrics and Gynecology, Osaka University Faculty of Medicine, Osaka, Japan. 25. Sanno Hospital, Tokyo, Japan. 26. Perinatal Medical Center, Tokyo Women's Medical University Hospital, Tokyo, Japan. 27. Department of Obstetrics and Gynecology, Tokai University School of Medicine, Kanagawa, Japan. 28. Department of Obstetrics and Gynecology, School of Medicine, Faculty of Medicine, Toho University, Tokyo, Japan. 29. Department of Obstetrics and Gynecology, Japanese Red Cross Medical Center, Tokyo, Japan. 30. Department of Obstetrics and Gynecology, Nara Medical University, Nara, Japan. 31. Department of Perinatology and Gynecology, National Cerebral and Cardiovascular Center, Osaka, Japan. 32. Department of Obstetrics and Gynecology, Okayama University Graduate School of Medicine, Okayama, Japan. 33. Department of Obstetrics and Gynecology, Hiroshima University Graduate School of Medicine, Hiroshima, Japan. 34. Department of Molecular Pathology, Faculty of Medicine, Oita University, Oita, Japan. 35. Department of Obstetrics and Gynecology, Kumamoto University, Kumamoto, Japan. 36. Department of Obstetrics and Gynecology, Showa University Northern Yokohama Hospital, Yokohama, Japan. 37. Departments of Obstetrics and Gynecology, Tokyo University Hospital, Tokyo, Japan. 38. Division of Clinical Genetics, Nippon Medical School Hospital, Tokyo, Japan. 39. Departments of Genetic Counseling, Ochanomizu University, Tokyo, Japan.
Abstract
OBJECTIVE: The purpose of this study is to compare the fetal fractions during non-invasive prenatal testing (NIPT) in singleton pregnancies according to gestational age and maternal characteristics to evaluate the utility of this parameter for the prediction of pregnancy complications including gestational diabetes mellitus (GDM) and hypertensive disorders of pregnancy (HDP). STUDY DESIGN: This study was a multicenter prospective cohort study. The present data were collected from women whose NIPT results were negative. The relationships between the fetal fractions and the gestational age, maternal weight and height, and incidences of miscarriage, preterm delivery, and pregnancy complications including GDM, HDP and placental abruption were assessed. RESULTS: A total of 5582 pregnant women with verified NIPT negative results were registered in the study. The demographic characteristics of the study populations were statistically analyzed, and the women with HDP tended to have a low fetal fraction in samples taken during early gestation. The area under the curve (AUC) in a receiver operating characteristic curve (ROC) analysis was 0.608 for women with HDP. CONCLUSION: A low fetal fraction on NIPT might be correlated with future HDP. However, predicting HDP during early pregnancy in women with a low fetal fraction might be difficult.
OBJECTIVE: The purpose of this study is to compare the fetal fractions during non-invasive prenatal testing (NIPT) in singleton pregnancies according to gestational age and maternal characteristics to evaluate the utility of this parameter for the prediction of pregnancy complications including gestational diabetes mellitus (GDM) and hypertensive disorders of pregnancy (HDP). STUDY DESIGN: This study was a multicenter prospective cohort study. The present data were collected from women whose NIPT results were negative. The relationships between the fetal fractions and the gestational age, maternal weight and height, and incidences of miscarriage, preterm delivery, and pregnancy complications including GDM, HDP and placental abruption were assessed. RESULTS: A total of 5582 pregnant women with verified NIPT negative results were registered in the study. The demographic characteristics of the study populations were statistically analyzed, and the women with HDP tended to have a low fetal fraction in samples taken during early gestation. The area under the curve (AUC) in a receiver operating characteristic curve (ROC) analysis was 0.608 for women with HDP. CONCLUSION: A low fetal fraction on NIPT might be correlated with future HDP. However, predicting HDP during early pregnancy in women with a low fetal fraction might be difficult.