Y A Mariño1, O E Ospina1,2, J C Verle Rodrigues2, P Bayman1. 1. Department of Biology, University of Puerto Rico - Río Piedras, San Juan, PR, USA. 2. Department of Agroenvironmental Sciences, Center for Excellence in Quarantine & Invasive Species, Agricultural Experimental Station - Río Piedras, University of Puerto Rico - Mayagüez, San Juan, PR, USA.
Abstract
AIMS: Variation in microbiota of the coffee berry borer (CBB) Hypothenemus hampei was studied. Diversity, structure and function of bacterial communities were compared between eggs vs adults, CBBs from shade coffee vs sun coffee, CBBs from the field vs raised in the laboratory, and CBBs with and without the antibiotic tetracycline. METHODS AND RESULTS: We sequenced the region V4 of the gene 16 S rRNA. Pseudomonadaceae and Enterobacteriaceae, particularly Pseudomonas and Pantoea, dominated microbiotas of the CBB. Comparative functional inferences with PICRUSt suggested that samples from the field were enriched for genes involved in carbohydrate and protein digestion and absorption, while laboratory-reared samples were higher in genes for melanization and caffeine metabolism. CONCLUSIONS: Microbiotas of the CBB were diverse and dominated by the genus Pseudomonas, several species of which have been previously associated with caffeine degradation in this insect. Wolbachia was the only endosymbiont detected with known ability to manipulate host reproduction. SIGNIFICANCE AND IMPACT OF THE STUDY: This study demonstrates that stage of development and origin of samples affected the structure and function of the CBB's bacterial communities. This is the first attempt to predict functional significance of the CBB microbiota in nutrition, reproduction and defence.
AIMS: Variation in microbiota of the coffee berry borer (CBB) Hypothenemus hampei was studied. Diversity, structure and function of bacterial communities were compared between eggs vs adults, CBBs from shade coffee vs sun coffee, CBBs from the field vs raised in the laboratory, and CBBs with and without the antibiotic tetracycline. METHODS AND RESULTS: We sequenced the region V4 of the gene 16 S rRNA. Pseudomonadaceae and Enterobacteriaceae, particularly Pseudomonas and Pantoea, dominated microbiotas of the CBB. Comparative functional inferences with PICRUSt suggested that samples from the field were enriched for genes involved in carbohydrate and protein digestion and absorption, while laboratory-reared samples were higher in genes for melanization and caffeine metabolism. CONCLUSIONS: Microbiotas of the CBB were diverse and dominated by the genus Pseudomonas, several species of which have been previously associated with caffeine degradation in this insect. Wolbachia was the only endosymbiont detected with known ability to manipulate host reproduction. SIGNIFICANCE AND IMPACT OF THE STUDY: This study demonstrates that stage of development and origin of samples affected the structure and function of the CBB's bacterial communities. This is the first attempt to predict functional significance of the CBB microbiota in nutrition, reproduction and defence.
Authors: Melissa A Johnson; Claudia Patricia Ruiz-Diaz; Nicholas C Manoukis; Jose Carlos Verle Rodrigues Journal: Insects Date: 2020-12-12 Impact factor: 2.769
Authors: Fernan Santiago Mejía-Alvarado; Thaura Ghneim-Herrera; Carmenza E Góngora; Pablo Benavides; Lucio Navarro-Escalante Journal: Front Microbiol Date: 2021-07-01 Impact factor: 5.640