Differential Modulation of Endophytic Microbiome of Ginger in the Presence of Beneficial Organisms, Pathogens and Both as Identified by DGGE Analysis.

Endophytic microorganisms play a significant role in plants response to beneficial organisms and pathogens. In the current study, endophytic microorganisms from Zingiber officinale were screened for in vitro inhibition against Pythium myriotylum. From this, Burkholderia vietnamiensis ZoB74 was selected as an organism with remarkable antifungal effect. Further, the study focussed on analysis of in vivo changes in endophytic bacterial community of Z. officinale in presence of selected organisms and the pathogen P. myriotylum by PCR-DGGE. 16S rDNA sequencing of bacterial community after DGGE has resulted in the identification of a group of uncultured bacteria as the predominant microbial community of rhizome under various conditions of treatment. High frequency dominance of these endophytic bacteria suggests their role in disease resistance to soft rot in ginger. This also revealed the variation of endophytic microbiome of Z. officinale under biotic stress. Hence the study provides molecular insight into uncultured microbiome and its stress-inducible variation in ginger rhizome.