Sabrina Schmid1, Eva Goldberg-Bockhorn2, Silke Schwarz2,3, Nicole Rotter2,4, Jan Kassubek1, Kelly Del Tredici1, Elmar Pinkhardt1, Markus Otto1, Albert C Ludolph5, Patrick Oeckl1. 1. Department of Neurology, University of Ulm, Oberer Eselsberg 45, Ulm, Germany. 2. Department of Otorhinolaryngology, Ulm University Hospital, Ulm, Germany. 3. Department of Anatomy, Paracelsus Medical University, Nuremberg, Germany. 4. Department of Otorhinolaryngology, University Medical Center Mannheim, Mannheim, Germany. 5. Department of Neurology, University of Ulm, Oberer Eselsberg 45, Ulm, Germany. albert.ludolph@rku.de.
Abstract
INTRODUCTION: In autopsy cases staged for sporadic Parkinson's disease (PD), the neuropathology is characterized by a preclinical phase that targets the enteric nervous system of the gastrointestinal tract (GIT). Therefore, the ENS might be a source of potential (presymptomatic) PD biomarkers. METHODS: In this clinically based study, we examined the alpha-synuclein (αSyn) concentration in an easily accessible protein storage medium of the GIT, dental calculus, in 21/50 patients with PD and 28/50 age- and gender-matched controls using ELISA. RESULTS: αSyn was detectable in dental calculus and the median concentration in the control patients was 8.6 pg/mg calculus (interquartile range 2.6-13.1 pg/mg). αSyn concentrations were significantly influenced by blood contamination and samples with a hemoglobin concentration of > 4000 ng/mL were excluded. There was no significant difference of αSyn concentrations in the dental calculus of PD patients (5.76 pg/mg, interquartile range 2.91-9.74 pg/mg) compared to those in controls (p = 0.40). CONCLUSION: The total αSyn concentration in dental calculus is not a suitable biomarker for sporadic PD. Disease-related variants such as oligomeric or phosphorylated αSyn in calculus might prove to be more specific.
INTRODUCTION: In autopsy cases staged for sporadic Parkinson's disease (PD), the neuropathology is characterized by a preclinical phase that targets the enteric nervous system of the gastrointestinal tract (GIT). Therefore, the ENS might be a source of potential (presymptomatic) PD biomarkers. METHODS: In this clinically based study, we examined the alpha-synuclein (αSyn) concentration in an easily accessible protein storage medium of the GIT, dental calculus, in 21/50 patients with PD and 28/50 age- and gender-matched controls using ELISA. RESULTS: αSyn was detectable in dental calculus and the median concentration in the control patients was 8.6 pg/mg calculus (interquartile range 2.6-13.1 pg/mg). αSyn concentrations were significantly influenced by blood contamination and samples with a hemoglobin concentration of > 4000 ng/mL were excluded. There was no significant difference of αSyn concentrations in the dental calculus of PDpatients (5.76 pg/mg, interquartile range 2.91-9.74 pg/mg) compared to those in controls (p = 0.40). CONCLUSION: The total αSyn concentration in dental calculus is not a suitable biomarker for sporadic PD. Disease-related variants such as oligomeric or phosphorylated αSyn in calculus might prove to be more specific.
Authors: Patrick Oeckl; Fabian Metzger; Magdalena Nagl; Christine A F von Arnim; Steffen Halbgebauer; Petra Steinacker; Albert C Ludolph; Markus Otto Journal: Mol Cell Proteomics Date: 2016-08-09 Impact factor: 5.911
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