Chung-Lin Tsai1,2, Ying-Ming Chiu3,4, Tin-Yun Ho5, Chin-Tung Hsieh6, Dong-Chen Shieh4, Yi-Ju Lee7, Gregory J Tsay8,9, Yi-Ying Wu10. 1. Division of Cardiovascular Surgery, Taichung Veterans General Hospital, Taichung, Taiwan, R.O.C. 2. Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan, R.O.C. 3. Division of Allergy, Immunology & Rheumatology, Changhua Christian Hospital, Changhua, Taiwan, R.O.C. 4. Department of Nursing, College of Medicine & Nursing, Hungkuang University, Taichung, Taiwan, R.O.C. 5. Graduate Institute of Chinese Medicine, China Medical University, Taichung, Taiwan, R.O.C. 6. Department of Pediatrics, Lotung Poh-Ai Hospital, I-Lan, Taiwan, R.O.C. 7. Institute of Biochemistry, Microbiology and Immunology, Chung Shan Medical University, Taichung, Taiwan, R.O.C. 8. Division of Immunology and Rheumatology, Department of Internal Medicine, China Medical University Hospital, Taichung, Taiwan, R.O.C. 9. School of Medicine, College of Medicine, China Medical University, Taichung, Taiwan, R.O.C. 10. Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan, R.O.C. yyw@mail.cmu.edu.tw.
Abstract
BACKGROUND/AIM: Gastric cancer is one of the most common malignant cancers with a poor prognosis and high mortality rate worldwide. Current treatment of gastric cancer includes surgery and chemotherapy as the main modalities, but the potentially severe side-effects of chemotherapy present a considerable challenge. Gallic acid is a trihydroxybenzoic acid found to exert an anticancer effect against a variety of cancer cells. The purpose of this study was to determine the anti-cancer activity of Galla chinensis and its main component gallic acid on human gastric adenocarcinoma cells. MATERIALS AND METHODS: MTT assay and cell death ELISA were used to determine the apoptotic effect of Gallic Chinensis and gallic acid on human gastric adenocarcinoma cells. To determine the pathway and relevant components by which gallic acid-induced apoptosis is mediated through, cells were transfected with siRNA (Fas, FasL, DR5, p53) using Lipofectamine 2000. Reults: Gallic Chinensis and gallic acid induced apoptosis of human gastric adenocarcinoma cells. Gallic acid induced up-regulation of Fas, FasL, and DR5 expression in AGS cells. Transfection of cells with Fas, FasL, or DR5 siRNA reduced gallic acid-induced cell death. In addition, p53 was shown to be involved in gallic acid-mediated Fas, FasL, and DR5 expression as well as cell apoptosis in AGS cells. CONCLUSION: These results suggest that gallic acid has a potential role in the treatment of gastric cancer. Copyright
BACKGROUND/AIM: Gastric cancer is one of the most common malignant cancers with a poor prognosis and high mortality rate worldwide. Current treatment of gastric cancer includes surgery and chemotherapy as the main modalities, but the potentially severe side-effects of chemotherapy present a considerable challenge. Gallic acid is a trihydroxybenzoic acid found to exert an anticancer effect against a variety of cancer cells. The purpose of this study was to determine the anti-cancer activity of Galla chinensis and its main component gallic acid on humangastric adenocarcinoma cells. MATERIALS AND METHODS:MTT assay and cell death ELISA were used to determine the apoptotic effect of Gallic Chinensis and gallic acid on humangastric adenocarcinoma cells. To determine the pathway and relevant components by which gallic acid-induced apoptosis is mediated through, cells were transfected with siRNA (Fas, FasL, DR5, p53) using Lipofectamine 2000. Reults: Gallic Chinensis and gallic acid induced apoptosis of humangastric adenocarcinoma cells. Gallic acid induced up-regulation of Fas, FasL, and DR5 expression in AGS cells. Transfection of cells with Fas, FasL, or DR5 siRNA reduced gallic acid-induced cell death. In addition, p53 was shown to be involved in gallic acid-mediated Fas, FasL, and DR5 expression as well as cell apoptosis in AGS cells. CONCLUSION: These results suggest that gallic acid has a potential role in the treatment of gastric cancer. Copyright