| Literature DB >> 2959526 |
H H Dieter1, L Müller, J Abel, K H Summer.
Abstract
Five current methods of metallothionein (MT)-estimation in biological materials (Hg/TCA-, Cd/heme-, SH-assay, G 75-method and RIA) were compared. Uniform calibration was ensured by measuring the nitrogen-content of the standard-apoprotein after "Kjeldahl-digestion". Known amounts of rabbit standard Cd-MT I were recovered from identical rat S9-samples. In an overall rating, RIA and Cd/heme-assay performed best, whereas the other methods either underestimated (SH-, G 75) or overestimated (Hg/TCA) the 100%-expectation.Entities:
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Year: 1987 PMID： 2959526 DOI： 10.1007/978-3-0348-6784-9_33
Source DB: PubMed Journal: Experientia Suppl ISSN： 0071-335X