| Literature DB >> 29589313 |
Paula Álvarez-Chaver1, Loretta De Chiara2, Vicenta Soledad Martínez-Zorzano2.
Abstract
Nowadays, the ideal biomarker for colorectal cancer (CRC) has not been found. Two-dimensional electrophoresis (2-DE) and mass spectrometry (MS) are suitable techniques for searching new biomarkers. In this chapter, we describe methodology for biomarker discovery based on a proteomic approach. In addition, special attention is given to the sample preparation, including protein extraction, fractionation, and cleanup, as we consider this a critical step. Comparing the proteomic profile of tumor and mucosa, we identified the nucleoside diphosphate kinase A (NDKA) protein as a candidate biomarker for CRC. Finally, we validated NDKA with an ELISA kit using serum samples from individuals of a screening cohort. Our results suggest that serum NDKA is a potential biomarker for screening of CRC and premalignant advanced adenomas (AA).Entities:
Keywords: Biomarkers; Colorectal cancer; ELISA immunoassay; Mass spectrometry; Nucleoside diphosphate kinase A; Proteomics; Serum; Tissue; Two-dimensional electrophoresis; Western blot
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Year: 2018 PMID： 29589313 DOI： 10.1007/978-1-4939-7765-9_16
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745