Jingyi Shao1, Amanda J Wheeler2, Ling Chen3, Bo Strandberg4, Andrea Hinwood5, Fay H Johnston1, Graeme R Zosky6. 1. Menzies Institute for Medical Research, University of Tasmania, Hobart, Tasmania 7000, Australia. 2. Menzies Institute for Medical Research, University of Tasmania, Hobart, Tasmania 7000, Australia; Edith Cowan University, 270 Joondalup Drive, Joondalup, Western Australia 6017, Australia. 3. School of Medicine, Faculty of Health, University of Tasmania, Hobart, Tasmania 7000, Australia. 4. Section of Occupational and Environmental Medicine, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden; Division of Occupational and Environmental Medicine, Lund University, Lund, Sweden. 5. Edith Cowan University, 270 Joondalup Drive, Joondalup, Western Australia 6017, Australia; Environmental Protection Authority Victoria, Carlton, Victoria 3053, Australia. 6. School of Medicine, Faculty of Health, University of Tasmania, Hobart, Tasmania 7000, Australia. Electronic address: Graeme.Zosky@utas.edu.au.
Abstract
BACKGROUND: Adverse health effects of particulate matter (PM) vary with chemical composition; however, evidence regarding which elements are the most detrimental is limited. The roof space area provides an open and stable environment for outdoor PM to settle and deposit. Therefore, this study used roof space PM samples as a proxy of residential cumulative exposure to outdoor air pollution to investigate their pro-inflammatory effects on human lung cells and the contribution of the endotoxin and chemical content. METHODS: Roof space PM samples of 36 different homes were collected and analysed using standardised techniques. We evaluated cytotoxicity and cytokine production of BEAS-2B cells after PM exposure using MTS and ELISA, respectively. Principle component analysis (PCA) and linear regression analyses were employed to assess the associations between cytokine production and the PM components. RESULTS: PM caused significant time- and dose-dependent increases in cellular cytokine production (p < 0.05). PCA identified four factors that explained 68.33% of the variance in the chemical composition. An increase in Factor 1 (+Fe, +Al, +Mn) score and a decrease in Factor 2 (-Ca, +Pb, +PAH) score were associated with increased interleukin (IL)-6 (Factor 1; p = 0.010; Factor 2; p = 0.006) and IL-8 (Factor 1; p = 0.003; Factor 2; p = 0.020) production, however, only the association with Factor 1 was evident after correcting for endotoxin and particle size. CONCLUSIONS: Our study provides novel insight into the positive associations between pro-inflammatory effects of roof space PM samples with Fe, Al and Mn levels.
BACKGROUND: Adverse health effects of particulate matter (PM) vary with chemical composition; however, evidence regarding which elements are the most detrimental is limited. The roof space area provides an open and stable environment for outdoor PM to settle and deposit. Therefore, this study used roof space PM samples as a proxy of residential cumulative exposure to outdoor air pollution to investigate their pro-inflammatory effects on human lung cells and the contribution of the endotoxin and chemical content. METHODS: Roof space PM samples of 36 different homes were collected and analysed using standardised techniques. We evaluated cytotoxicity and cytokine production of BEAS-2B cells after PM exposure using MTS and ELISA, respectively. Principle component analysis (PCA) and linear regression analyses were employed to assess the associations between cytokine production and the PM components. RESULTS: PM caused significant time- and dose-dependent increases in cellular cytokine production (p < 0.05). PCA identified four factors that explained 68.33% of the variance in the chemical composition. An increase in Factor 1 (+Fe, +Al, +Mn) score and a decrease in Factor 2 (-Ca, +Pb, +PAH) score were associated with increased interleukin (IL)-6 (Factor 1; p = 0.010; Factor 2; p = 0.006) and IL-8 (Factor 1; p = 0.003; Factor 2; p = 0.020) production, however, only the association with Factor 1 was evident after correcting for endotoxin and particle size. CONCLUSIONS: Our study provides novel insight into the positive associations between pro-inflammatory effects of roof space PM samples with Fe, Al and Mn levels.
Authors: M Viana; A Salmatonidis; S Bezantakos; C Ribalta; N Moreno; P Córdoba; F R Cassee; J Boere; S Fraga; J P Teixeira; M J Bessa; E Monfort Journal: Ann Work Expo Health Date: 2021-10-09 Impact factor: 2.179