Daniel Tran1,2, Aurélien Dauphin1,3, Patrice Meimoun1,4, Takashi Kadono1,5, Hieu T H Nguyen6, Delphine Arbelet-Bonnin1, Tingting Zhao1, Rafik Errakhi1,7, Arnaud Lehner1,8, Tomonori Kawano6,9,10, François Bouteau1,9. 1. Université Paris Diderot, Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain, Paris, France. 2. Department of Physiology & Cell Information Systems Group, McGill University, Montréal, Québec, Canada. 3. Institut Curie, CNRS UMR3215, INSERM U934, Paris, France. 4. Sorbonne Université, UMR7622-IBPS, Paris, France. 5. Laboratory of Aquatic Environmental Science, Kochi University, Kochi, Japan. 6. Graduate School of Environmental Engineering, University of Kitakyushu, Wakamatsu-ku, Kitakyushu, Japan. 7. Eurofins Agriscience Service, Marocco. 8. Normandie Université, UNIROUEN, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale, EA4358, SFR Normandie végétal, Rouen, France. 9. LINV Kitakyushu Research Center, Kitakyushu, Japan. 10. Université Paris Diderot, Sorbonne Paris Cité, Paris Interdisciplinary Energy Research Institute (PIERI), Paris, France.
Abstract
Background and Aims: Methanol is a volatile organic compound released from plants through the action of pectin methylesterases (PMEs), which demethylesterify cell wall pectins. Plant PMEs play a role in developmental processes but also in responses to herbivory and infection by fungal or bacterial pathogens. However, molecular mechanisms that explain how methanol could affect plant defences remain poorly understood. Methods: Using cultured cells and seedlings from Arabidopsis thaliana and tobacco BY2 expressing the apoaequorin gene, allowing quantification of cytosolic Ca2+, a reactive oxygen species (ROS) probe (CLA, Cypridina luciferin analogue) and electrophysiological techniques, we followed early plant cell responses to exogenously supplied methanol applied as a liquid or as volatile. Key Results: Methanol induces cytosolic Ca2+ variations that involve Ca2+ influx through the plasma membrane and Ca2+ release from internal stores. Our data further suggest that these Ca2+ variations could interact with different ROS and support a signalling pathway leading to well known plant responses to pathogens such as plasma membrane depolarization through anion channel regulation and ethylene synthesis. Conclusions: Methanol is not only a by-product of PME activities, and our data suggest that [Ca2+]cyt variations could participate in signalling processes induced by methanol upstream of plant defence responses.
Background and Aims: Methanol is a volatile organic compound released from plants through the action of pectin methylesterases (PMEs), which demethylesterify cell wall pectins. Plant PMEs play a role in developmental processes but also in responses to herbivory and infection by fungal or bacterial pathogens. However, molecular mechanisms that explain how methanol could affect plant defences remain poorly understood. Methods: Using cultured cells and seedlings from Arabidopsis thaliana and tobaccoBY2 expressing the apoaequorin gene, allowing quantification of cytosolic Ca2+, a reactive oxygen species (ROS) probe (CLA, Cypridina luciferin analogue) and electrophysiological techniques, we followed early plant cell responses to exogenously supplied methanol applied as a liquid or as volatile. Key Results:Methanol induces cytosolic Ca2+ variations that involve Ca2+ influx through the plasma membrane and Ca2+ release from internal stores. Our data further suggest that these Ca2+ variations could interact with different ROS and support a signalling pathway leading to well known plant responses to pathogens such as plasma membrane depolarization through anion channel regulation and ethylene synthesis. Conclusions: Methanol is not only a by-product of PME activities, and our data suggest that [Ca2+]cyt variations could participate in signalling processes induced by methanol upstream of plant defence responses.
Authors: Ian T Baldwin; Rayko Halitschke; Anja Paschold; Caroline C von Dahl; Catherine A Preston Journal: Science Date: 2006-02-10 Impact factor: 47.728