Kinetics of the beta-thromboglobulin release from alpha-granules of blood platelets activated by ADP.

Mathematical model of the beta-thromboglobulin (BTG) release from alpha-granules stimulated with ADP is compared with experimental data. Secretion of BTG was analysed using platelets washed by different procedures in the presence or absence of inhibitors of platelet activation. The release of BTG was monitored in response to 5-100 microM ADP. The rate constant of the release of BTG (K = 1.83 X 10(3) M-1 min-1) and efficient amount of ADP molecules secreted by a single platelet (W = 5.2 X 10(-18) mole) were estimated. The model precisely describes the release of BTG associated with the isolation of platelets (blood collection, washing procedure) and allows to estimate the extent of activation of blood platelets in the suspension.