Mustafa Ufuk Uylaş1, Adnan Şahin2, Varol Şahintürk3, İbrahim Özkan Alataş4. 1. Department of General Surgery, Turgut Ozal Medical Center, Inonu University, Malatya, Turkey. Electronic address: ufukuylas@hotmail.com. 2. Department of General Surgery, Eskisehir Osmangazi University, Eskisehir, Turkey. Electronic address: asahin@ogu.edu.tr. 3. Department of Histology and Embryology, Eskisehir Osmangazi University, Eskisehir, Turkey. Electronic address: varols@ogu.edu.tr. 4. Department of Medical Biochemistry, Eskisehir Osmangazi University, Eskisehir, Turkey. Electronic address: oalatas@ogu.edu.tr.
Abstract
BACKGROUND: Quercetin found in fruits and vegetables has an antioxidative effect. We aimed to investigate the protective effects of quercetin according to different doses on hepatic and ischemia-reperfusion (I/R) injury. METHODS: Fifty mature male Sprague-Dawley rats were randomly divided into five groups (n = 10 for each). All the animal groups underwent laparotomy. Group 1 rats served as a sham-operated group. Groups 2-5 underwent 1 h hepatic ischemia and were followed by 2 h reperfusion. Group 3-5 animals received an additional intraperitoneal dose of 25, 50 or 100 mg/kg quercetin respectively before I/R operation. Blood samples were collected for determining serum aspartate transaminase (AST), alanine transaminase (ALT) and malondialdehyde (MDA) levels. Also, liver tissue samples were taken for measuring of liver MDA concentration and for histopathology assessment. RESULTS: The highest levels of biochemical parameters were observed in group 2. In quercetin-treated groups, serum AST, ALT, MDA levels, and tissue MDA concentration were decreased as inversely with increasing quercetin dose. Microscopic evaluation revealed that most conspicuous histological improvement was observed in 50 mg/kg quercetin co-treated rats. 25 and 100 mg/kg quercetin co-treatment could not protect completely against hepatic I/R injury. CONCLUSION: Quercetin can be effective in preventing of hepatic I/R injury when the correct dose was used.
BACKGROUND:Quercetin found in fruits and vegetables has an antioxidative effect. We aimed to investigate the protective effects of quercetin according to different doses on hepatic and ischemia-reperfusion (I/R) injury. METHODS: Fifty mature male Sprague-Dawley rats were randomly divided into five groups (n = 10 for each). All the animal groups underwent laparotomy. Group 1 rats served as a sham-operated group. Groups 2-5 underwent 1 h hepatic ischemia and were followed by 2 h reperfusion. Group 3-5 animals received an additional intraperitoneal dose of 25, 50 or 100 mg/kg quercetin respectively before I/R operation. Blood samples were collected for determining serum aspartate transaminase (AST), alanine transaminase (ALT) and malondialdehyde (MDA) levels. Also, liver tissue samples were taken for measuring of liver MDA concentration and for histopathology assessment. RESULTS: The highest levels of biochemical parameters were observed in group 2. In quercetin-treated groups, serum AST, ALT, MDA levels, and tissue MDA concentration were decreased as inversely with increasing quercetin dose. Microscopic evaluation revealed that most conspicuous histological improvement was observed in 50 mg/kg quercetin co-treated rats. 25 and 100 mg/kg quercetin co-treatment could not protect completely against hepatic I/R injury. CONCLUSION:Quercetin can be effective in preventing of hepatic I/R injury when the correct dose was used.
Authors: Georgios Kyriakopoulos; Georgia Valsami; Christos Tsalikidis; Michail Pitiakoudis; Alexandra K Tsaroucha Journal: Ann Med Surg (Lond) Date: 2020-11-26
Authors: Margarida Ferreira-Silva; Catarina Faria-Silva; Manuela C Carvalheiro; Sandra Simões; H Susana Marinho; Paulo Marcelino; Maria Celeste Campos; Josbert M Metselaar; Eduarda Fernandes; Pedro V Baptista; Alexandra R Fernandes; Maria Luísa Corvo Journal: Pharmaceutics Date: 2022-01-03 Impact factor: 6.321