E Bonin1, A R Dos Santos1, A Fiori da Silva2, L H Ribeiro3, M E Favero4, P A Z Campanerut-Sá5, C F de Freitas6, W Caetano6,7, N Hioka6,7, J M G Mikcha1,2,5. 1. Programa de Pós-Graduação em Ciência de Alimentos, Universidade Estadual de Maringá, Maringá, PR, Brazil. 2. Programa de Pós-Graduação em Ciências da Saúde, Universidade Estadual de Maringá, Maringá, PR, Brazil. 3. Curso de Engenharia de Alimentos, Departamento de Engenharia de Alimentos, Universidade Estadual de Maringá, Maringá, PR, Brazil. 4. Departamento de Patologia, Análises Clínicas e Toxicológicas, Universidade Estadual de Londrina, Londrina, PR, Brazil. 5. Departamento de Análises Clínicas e Biomedicina, Universidade Estadual de Maringá, Maringá, PR, Brazil. 6. Programa de Pós-Graduação em Química, Departamento de Química, Universidade Estadual de Maringá, Maringá, PR, Brazil. 7. Departamento de Química, Universidade Estadual de Maringá, Maringá, PR, Brazil.
Abstract
AIMS: The aim of this study was evaluate the effect of photodynamic inactivation mediated by eosin Y in Salmonella enterica serotype Typhimurium ATCC 14028, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923 and Bacillus cereus ATCC 11778. METHODS AND RESULTS: Bacteria (107 CFU per ml) were incubated with eosin Y at concentrations ranging from 0·1 to 10 μmol l-1 , irradiated by green LED (λmax 490-570 nm) for 5, 10 and 15 min and the cellular viability was determined. Pseudomonas aeruginosa was completely inactivated when treated with 10 μmol l-1 eosin Y for 10 min. Treatments reduced B. cereus and Salm. Typhimurium counts to 2·7 log CFU per ml and 1·7 log CFU per ml, respectively. Escherichia coli counts were slightly reduced. Staphylococcus aureus presented the highest sensitivity, being completely inactivated by eosin Y at 5 μmol l-1 and 5 min of illumination. The reduction of cellular viability of photoinactivated Staph. aureus was also demonstrated by flow cytometry and morphological changes were observed by scanning electron microscopy. CONCLUSIONS: Eosin Y in combination with LED produced bacterial inactivation, being a potential candidate for photodynamic inactivation. SIGNIFICANCE AND IMPACT OF THE STUDY: This study evidenced the efficacy of photodynamic inactivation as a novel and promising alternative to bacterial control.
AIMS: The aim of this study was evaluate the effect of photodynamic inactivation mediated by eosin Y in Salmonella enterica serotype Typhimurium ATCC 14028, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923 and Bacillus cereus ATCC 11778. METHODS AND RESULTS: Bacteria (107 CFU per ml) were incubated with eosin Y at concentrations ranging from 0·1 to 10 μmol l-1 , irradiated by green LED (λmax 490-570 nm) for 5, 10 and 15 min and the cellular viability was determined. Pseudomonas aeruginosa was completely inactivated when treated with 10 μmol l-1 eosin Y for 10 min. Treatments reduced B. cereus and Salm. Typhimurium counts to 2·7 log CFU per ml and 1·7 log CFU per ml, respectively. Escherichia coli counts were slightly reduced. Staphylococcus aureus presented the highest sensitivity, being completely inactivated by eosin Y at 5 μmol l-1 and 5 min of illumination. The reduction of cellular viability of photoinactivated Staph. aureus was also demonstrated by flow cytometry and morphological changes were observed by scanning electron microscopy. CONCLUSIONS:Eosin Y in combination with LED produced bacterial inactivation, being a potential candidate for photodynamic inactivation. SIGNIFICANCE AND IMPACT OF THE STUDY: This study evidenced the efficacy of photodynamic inactivation as a novel and promising alternative to bacterial control.
Authors: Adriele R Santos; Andréia F P Batista; Ana T P C Gomes; Maria da Graça P M S Neves; Maria Amparo F Faustino; Adelaide Almeida; Noboru Hioka; Jane M G Mikcha Journal: Antibiotics (Basel) Date: 2019-11-05
Authors: Adriele R Santos; Alex F da Silva; Andréia F P Batista; Camila F Freitas; Evandro Bona; Maria J Sereia; Wilker Caetano; Noburu Hioka; Jane M G Mikcha Journal: Antibiotics (Basel) Date: 2020-03-17