Sulsal-Ul Haque1, Liang Niu2, Damaris Kuhnell2, Jacob Hendershot2, Jacek Biesiada2, Wen Niu2, Matthew C Hagan3, Karl T Kelsey4,5, Keith A Casper6, Trisha M Wise-Draper1,7, Mario Medvedovic2, Scott M Langevin2. 1. Department of Internal Medicine, Division of Hematology/Oncology, University of Cincinnati College of Medicine, Cincinnati, Ohio. 2. Department of Environmental Health, University of Cincinnati College of Medicine, Cincinnati, Ohio. 3. Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, Ohio. 4. Department of Epidemiology, Brown University, Providence, Rhode Island. 5. Department of Pathology and Laboratory Medicine, Brown University, Providence, Rhode Island. 6. Department of Otolaryngology, University of Michigan, Ann Arbor, Michigan. 7. Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, Ohio.
Abstract
BACKGROUND: Long non-coding RNA (lncRNA) has emerged as a new avenue of interest due to its various biological functions in cancer. Abnormal expression of lncRNA has been reported in other malignancies but has been understudied in head and neck squamous cell carcinoma (HNSCC). METHODS: The lncRNA expression was interrogated via quantitative real-time polymerase chain reaction (qRT-PCR) array for 19 human papillomavirus (HPV)-negative HNSCC tumor-normal pairs. The Cancer Genome Atlas (TCGA) was used to validate these results. The association between differentially expressed lncRNA and survival outcomes was analyzed. RESULTS: Differential expression was validated for 5 lncRNA (SPRY4-IT1, HEIH, LUCAT1, LINC00152, and HAND2-AS1). There was also an inverse association between MEG3 expression (not significantly differentially expressed in TCGA tumors but highly variable expression) and 3-year recurrence-free survival (RFS). CONCLUSION: We identified and validated differential expression of 5 lncRNA in HPV-negative HNSCC. Low MEG3 expression was associated with favorable 3-year RFS, although the significance of this finding remains unclear.
BACKGROUND: Long non-coding RNA (lncRNA) has emerged as a new avenue of interest due to its various biological functions in cancer. Abnormal expression of lncRNA has been reported in other malignancies but has been understudied in head and neck squamous cell carcinoma (HNSCC). METHODS: The lncRNA expression was interrogated via quantitative real-time polymerase chain reaction (qRT-PCR) array for 19 human papillomavirus (HPV)-negative HNSCC tumor-normal pairs. The Cancer Genome Atlas (TCGA) was used to validate these results. The association between differentially expressed lncRNA and survival outcomes was analyzed. RESULTS: Differential expression was validated for 5 lncRNA (SPRY4-IT1, HEIH, LUCAT1, LINC00152, and HAND2-AS1). There was also an inverse association between MEG3 expression (not significantly differentially expressed in TCGA tumors but highly variable expression) and 3-year recurrence-free survival (RFS). CONCLUSION: We identified and validated differential expression of 5 lncRNA in HPV-negative HNSCC. Low MEG3 expression was associated with favorable 3-year RFS, although the significance of this finding remains unclear.
Keywords:
The Cancer Genome Atlas (TCGA); head and neck cancer; head and neck squamous cell carcinoma (HNSCC); long noncoding RNA (lncRNA); noncoding RNA (ncRNA); survival
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