Fang Shi1, Zhenzhen Liang2, Qian Zhang3, Chunpeng Wang4, Xin Liu5. 1. Department of Epidemiology and Statistics, School of Public Health, Jilin University, Changchun 130021, China. Electronic address: shifang16@mails.jlu.edu.cn. 2. Department of Epidemiology and Statistics, School of Public Health, Jilin University, Changchun 130021, China. Electronic address: liangzz15@mails.jlu.edu.cn. 3. Department of Epidemiology and Statistics, School of Public Health, Jilin University, Changchun 130021, China. Electronic address: zhangqian15@mails.jlu.edu.cn. 4. School of Mathematics and Statistics, Northeast Normal University, Changchun, Jilin, China. Electronic address: wangcpnenu@163.com. 5. Department of Epidemiology and Statistics, School of Public Health, Jilin University, Changchun 130021, China. Electronic address: liuxinjlu01@163.com.
Abstract
BACKGROUND: One-step nucleic acid amplification (OSNA) assay is a molecular diagnostic method for intraoperative detection of lymph node metastasis by amplifying cytokeratin 19 mRNA. We aim to further evaluate the performance of OSNA assay for the intraoperative diagnosis of sentinel lymph node metastasis in breast cancer. METHODS: PubMed, Cochrane Library, Web of Science databases were searched to retrieve related literature published up until December 2017. This meta-analysis was performed using a random-effects model. Risks of bias and quality assessments of included studies were evaluated and subgroup analysis was performed. RESULTS: Nineteen studies were included in this meta-analysis. For overall metastasis, the pooled sensitivity, specificity and area under the summary receiver-operating characteristic curve (AUC) were 0.90, 0.96 and 0.98, respectively. For macrometastasis, the pooled sensitivity, specificity and AUC were 0.85, 0.98 and 0.94, respectively. CONCLUSION: OSNA assay is an accurate molecular diagnosis for intraoperative detection of sentinel lymph node macrometastasis in breast cancer.
BACKGROUND: One-step nucleic acid amplification (OSNA) assay is a molecular diagnostic method for intraoperative detection of lymph node metastasis by amplifying cytokeratin 19 mRNA. We aim to further evaluate the performance of OSNA assay for the intraoperative diagnosis of sentinel lymph node metastasis in breast cancer. METHODS: PubMed, Cochrane Library, Web of Science databases were searched to retrieve related literature published up until December 2017. This meta-analysis was performed using a random-effects model. Risks of bias and quality assessments of included studies were evaluated and subgroup analysis was performed. RESULTS: Nineteen studies were included in this meta-analysis. For overall metastasis, the pooled sensitivity, specificity and area under the summary receiver-operating characteristic curve (AUC) were 0.90, 0.96 and 0.98, respectively. For macrometastasis, the pooled sensitivity, specificity and AUC were 0.85, 0.98 and 0.94, respectively. CONCLUSION: OSNA assay is an accurate molecular diagnosis for intraoperative detection of sentinel lymph node macrometastasis in breast cancer.
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