| Literature DB >> 29571457 |
Anatoly P Sobolev1, Luisa Mannina2, Donatella Capitani1, Gabriella Sanzò3, Cinzia Ingallina3, Bruno Botta3, Simonetta Fornarini3, Maria Elisa Crestoni3, Barbara Chiavarino3, Simone Carradori4, Marcello Locatelli4, Anna Maria Giusti5, Giovanna Simonetti6, Giuliana Vinci7, Raffaella Preti7, Chiara Toniolo8, Massimo Reverberi8, Marzia Scarpari8, Alessia Parroni8, Lorena Abete9, Fausta Natella10, Antonella Di Sotto9.
Abstract
A multi-methodological approach was applied to study red sweet peppers (Capsicum annuum L.) ecotype "Cornetto di Pontecorvo" grown in a greenhouse or in open field. This approach includes morphological analysis, chemical composition determination, and biological activity evaluation of different extracts from pepper fruits. Untargeted analyses, namely NMR spectroscopy and mass spectrometry, allowed the comprehensive pepper metabolite profile of pepper pulp, peel and seeds hydroalcoholic and organic extracts to be determined, showing the presence of sugars, organic acids, amino acids and other secondary metabolites. Targeted analyses, such as HPLC-PDA, HPLC-TLC and spectrophotometric analyses allowed polyphenols, tannins, flavonoids and pigments content to be determined. Samples quality and freshness were verified by the low content of biogenic amines and mycotoxins, as determined using HPLC-FLD and HPLC-MS, respectively. Preliminary biological results demonstrated the ability of the organic extracts to inhibit α-amylase, a key enzyme in the control of glucose metabolism.Entities:
Keywords: Biogenic amines; HPLC; Mass spectrometry; Mycotoxins; NMR metabolite profile; Red sweet pepper; α-Amylase inhibition
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Year: 2018 PMID： 29571457 DOI： 10.1016/j.foodchem.2018.02.050
Source DB: PubMed Journal: Food Chem ISSN： 0308-8146 Impact factor: 7.514