| Literature DB >> 29564785 |
Sandeep Kumar1, Alyssia Lambert2, Jon Rainier2, Yingbin Fu3.
Abstract
Synthetic peptides derived from transmembrane segments of G protein-coupled receptors (GPCR) are used to disrupt GPCR dimer interface. This peptide competition technique is an effective approach to map the dimer interface of GPCR and its functional significance. Here we present a technique to deliver synthetic transmembrane peptides to living mouse rod photoreceptors to disrupt rhodopsin (a prototypical member of Class A GPCRs) dimer formation in the endoplasmic reticulum (ER). We have shown that rhodopsin helix H1- or H8-peptide caused mislocalization of rhodopsin to the perinuclear endoplasmic reticulum (ER).Entities:
Keywords: Cone opsin; Dimer interface; Dimerization; G protein-coupled receptor (GPCR); Nanoparticle delivery; Peptide competition; Protein trafficking; Rhodopsin; Rhodopsin dimer; Rhodopsin helix peptides
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Year: 2018 PMID： 29564785 PMCID： PMC5984041 DOI： 10.1007/978-1-4939-7720-8_8
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745