Agnes A Michalczyk1, Edward D Janus2,3, Alisha Judge1, Peter R Ebeling4, James D Best5, Michael J Ackland6, Dino Asproloupos7, James A Dunbar7, M Leigh Ackland1. 1. Centre for Cellular & Molecular Biology, School of Life & Environmental Sciences, Deakin University, Burwood, Victoria 3125, Australia. 2. University of Melbourne, Western Centre for Health Research & Education, Western Health, St Albans VIC 3021, Australia. 3. General Internal Medicine Unit, Western Health, Sunshine Hospital, St Albans, VIC 3021, Australia. 4. Department of Medicine, School of Clinical Sciences, Faculty of Medicine, Nursing & Health Sciences, Monash University, Victoria 3168, Australia. 5. Lee Kong Chian School of Medicine, Nanyang Technological University, 308232, Singapore. 6. The Alfred Centre, Monash University, Melbourne, Victoria 3004, Australia. 7. Centre for Population Health Research, Faculty of Health, Deakin University, Burwood, Victoria 3125, Australia.
Abstract
AIM: To investigate epigenomic changes in pregnancy and early postpartum in women with and without type 2 diabetes. METHODS: Dimethylation of histones H3K4, H3K9, H3K27, H3K36 and H3K79 was measured in white blood cells of women at 30 weeks pregnancy, at 8-10 and 20 weeks postpartum and in never-pregnant women. RESULTS: Dimethylation levels of all five histones were different between women in pregnancy and early postpartum compared with never-pregnant women and were different between women with and without type 2 diabetes. CONCLUSION: Histone methylation changes are transient in pregnancy and early postpartum and may represent normal physiological responses to hormones. Different epigenomic profiles in women with type 2 diabetes mellitus may correlate with hormonal responses, leading to high risk pregnancy outcomes.
AIM: To investigate epigenomic changes in pregnancy and early postpartum in women with and without type 2 diabetes. METHODS: Dimethylation of histones H3K4, H3K9, H3K27, H3K36 and H3K79 was measured in white blood cells of women at 30 weeks pregnancy, at 8-10 and 20 weeks postpartum and in never-pregnant women. RESULTS: Dimethylation levels of all five histones were different between women in pregnancy and early postpartum compared with never-pregnant women and were different between women with and without type 2 diabetes. CONCLUSION: Histone methylation changes are transient in pregnancy and early postpartum and may represent normal physiological responses to hormones. Different epigenomic profiles in women with type 2 diabetes mellitus may correlate with hormonal responses, leading to high risk pregnancy outcomes.
Entities:
Keywords:
epigenetics; histone methylation; pregnancy; type 2 diabetes mellitus
Authors: David A Bulger; Jermaine Conley; Spencer H Conner; Gipsy Majumdar; Solomon S Solomon Journal: Biochem Biophys Res Commun Date: 2015-04-24 Impact factor: 3.575
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