PURPOSE: To investigate the differential gene expression pattern between invasive multifocal/multicentric (MMBC) and unifocal breast cancer (UFBC) with cDNA array and to discover the potential outlier genes associated with the incidence of MMBC and also to provide a guidance for clinical treatment and prognosis prediction. METHODS: This retrospective study analyzed the gene expression pattern alteration in breast cancer. We collected 156 MMBC (136 cases with 2 foci, 20 cases with 3 foci) and 130 UFBC samples from patients hospitalized in Yuhuangding Hospital, Yantai, from January 2005 to December 2015. The outlier genes were screened by cDNA expression microarray and validated by RT-PCR. RESULTS: 18 overexpressed and 22 underexpressed genes were identified in the differential analysis, including family genes ABCC11, ABCB5 and PRODH, PROL1. Noteworthily, ABCC11 was significantly upregulated, while ABCB3 was downregulated, which were confirmed by RT-PCR results. CONCLUSION: The differential expression pattern of ABCC11 and ABCB5 genes may serve as outliers, potentially associated with incidence of MMBS.
PURPOSE: To investigate the differential gene expression pattern between invasive multifocal/multicentric (MMBC) and unifocal breast cancer (UFBC) with cDNA array and to discover the potential outlier genes associated with the incidence of MMBC and also to provide a guidance for clinical treatment and prognosis prediction. METHODS: This retrospective study analyzed the gene expression pattern alteration in breast cancer. We collected 156 MMBC (136 cases with 2 foci, 20 cases with 3 foci) and 130 UFBC samples from patients hospitalized in Yuhuangding Hospital, Yantai, from January 2005 to December 2015. The outlier genes were screened by cDNA expression microarray and validated by RT-PCR. RESULTS: 18 overexpressed and 22 underexpressed genes were identified in the differential analysis, including family genes ABCC11, ABCB5 and PRODH, PROL1. Noteworthily, ABCC11 was significantly upregulated, while ABCB3 was downregulated, which were confirmed by RT-PCR results. CONCLUSION: The differential expression pattern of ABCC11 and ABCB5 genes may serve as outliers, potentially associated with incidence of MMBS.