More and enhanced glyphosate analysis is needed.

Glyphosate is the world's most heavily applied herbicide. Worldwide, re-approval processes by authorities are ongoing, accompanied by intense public and political discussions on its possible carcinogenic effects. Further aspects involve human dietary exposure, its fate in the environment, and its impact on ecosystems. Many of these aspects are not yet fully understood. In many instances, the analytical strategies developed and applied so far have strong limitations, given the challenging physicochemical characteristics of glyphosate and its metabolites. Analytical chemists are still faced with problems in method development, reachable precision, and detection limits. Thus, not all open research questions can be answered with current strategies. This feature article wishes to address further needs in glyphosate analysis to foster enhancement of analytical strategies.