Literature DB >> 29552595

Obtaining Acute Brain Slices.

Thomas Papouin1, Philip G Haydon1.   

Abstract

Obtaining acute brain slices for electrophysiology or amperometric recordings has become a routine procedure in most labs in the field of neuroscience. Yet, protocols describing the step by step process are scarce, in particular for routine acute preparations such as from the mouse hippocampus. Here we provide a detailed protocol for the dissection, extraction and acute slicing of the mouse brain, including tips and list of material required.

Entities:  

Keywords:  Acute brain slices; Brain extraction; Dissection; Electrophysiology; Hippocampus; Mouse

Year:  2018        PMID： 29552595      PMCID： PMC5856250          DOI： 10.21769/BioProtoc.2699

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  3 in total

1.  Septal Cholinergic Neuromodulation Tunes the Astrocyte-Dependent Gating of Hippocampal NMDA Receptors to Wakefulness.

Authors:  Thomas Papouin; Jaclyn M Dunphy; Michaela Tolman; Kelly T Dineley; Philip G Haydon
Journal:  Neuron       Date:  2017-05-04       Impact factor: 17.173

2.  An Acute Mouse Spinal Cord Slice Preparation for Studying Glial Activation ex vivo.

Authors:  Juan Mauricio Garré; Guang Yang; Feliksas F Bukauskas; Michael V L Bennett
Journal:  Bio Protoc       Date:  2017-01-20

3.  D-serine Measurements in Brain Slices or Other Tissue Explants.

Authors:  Thomas Papouin; Philip G Haydon
Journal:  Bio Protoc       Date:  2018-01-20
  3 in total
  9 in total

1.  Pericyte Mapping in Cerebral Slices with the Far-red Fluorophore TO-PRO-3.

Authors:  Sandra P Mai-Morente; Juan P Irigoyen; Victoria M Carriquiry; Virginia M Marset; Mariana Di Doménico; Eugenia Isasi; Verónica Abudara
Journal:  Bio Protoc       Date:  2021-11-20

2.  Measurement of Dopamine Using Fast Scan Cyclic Voltammetry in Rodent Brain Slices.

Authors:  Madelyn I Mauterer; Paige M Estave; Katherine M Holleran; Sara R Jones
Journal:  Bio Protoc       Date:  2018-10-05

3.  ATAC-seq on Sorted Adult Mouse Neurons.

Authors:  Erin A Clark; Yasuyuki Shima; Sacha Nelson
Journal:  Bio Protoc       Date:  2019-10-05

4.  Hippocampal Unicellular Recordings and Hippocampal-dependent Innate Behaviors in an Adolescent Mouse Model of Alzheimer's disease.

Authors:  Siddhartha Mondragón-Rodríguez; Benito Ordaz; Erika Orta-Salazar; Sofia Díaz-Cintra; Fernando Peña-Ortega; George Perry
Journal:  Bio Protoc       Date:  2020-02-20

5.  Acute Lymph Node Slices Are a Functional Model System to Study Immunity Ex Vivo.

Authors:  Maura C Belanger; Alexander G Ball; Megan A Catterton; Andrew W L Kinman; Parastoo Anbaei; Benjamin D Groff; Stephanie J Melchor; John R Lukens; Ashley E Ross; Rebecca R Pompano
Journal:  ACS Pharmacol Transl Sci       Date:  2021-01-08

6.  D-serine Measurements in Brain Slices or Other Tissue Explants.

Authors:  Thomas Papouin; Philip G Haydon
Journal:  Bio Protoc       Date:  2018-01-20

7.  Immunofluorescence of GFAP and TNF-α in the Mouse Hypothalamus.

Authors:  Prasad S Dalvi; Denise D Belsham
Journal:  Bio Protoc       Date:  2021-07-05

8.  BACE1 partial deletion induces synaptic plasticity deficit in adult mice.

Authors:  Sylvia Lombardo; Martina Chiacchiaretta; Andrew Tarr; WonHee Kim; Tingyi Cao; Griffin Sigal; Thomas W Rosahl; Weiming Xia; Philip G Haydon; Matthew E Kennedy; Giuseppina Tesco
Journal:  Sci Rep       Date:  2019-12-27       Impact factor: 4.379

9.  Wolframin-1-expressing neurons in the entorhinal cortex propagate tau to CA1 neurons and impair hippocampal memory in mice.

Authors:  Jean-Christophe Delpech; Dhruba Pathak; Merina Varghese; Srinidhi Venkatesan Kalavai; Emma C Hays; Patrick R Hof; W Evan Johnson; Seiko Ikezu; Maria Medalla; Jennifer I Luebke; Tsuneya Ikezu
Journal:  Sci Transl Med       Date:  2021-09-15       Impact factor: 17.956
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.